PMID- 12387458
OWN - NLM
STAT- MEDLINE
DCOM- 20021107
LR  - 20190513
IS  - 0022-3069 (Print)
IS  - 0022-3069 (Linking)
VI  - 61
IP  - 10
DP  - 2002 Oct
TI  - Interphase cytogenetic analysis of lymphoma-associated chromosomal breakpoints in 
      primary diffuse large B-cell lymphomas of the central nervous system.
PG  - 926-33
AB  - Primary central nervous system lymphomas (PCNSLs) are germinal center-derived 
      diffuse large B-cell lymphomas (DLBCLs) arising in and remaining confined to the 
      brain, the pathogenesis of which is poorly understood. We investigated 13 PCNSLs 
      from immunocompetent patients by means of interphase cytogenetics on 
      cryopreserved cells derived from stereotactic biopsies. Interphase fluorescence 
      in situ hybridization (FISH) was performed for the detection of structural 
      alterations affecting the IGH (14q32), IGK (2p12), IGL (22q11), BCL6 (3q27), MYC 
      (8q24), CCND1 (11q13), MLT, and BCL2 (both 18q21) loci. Signal constellations 
      indicating breakpoints within the IGH and IGK locus were detected in 5 and 1 
      PCNSLs, respectively. There was no evidence for a t(8;14), t(11;14), or t(14;18) 
      in this series of tumors. Breakpoints in the BCL6 locus were observed in 3 of the 
      13 cases, and nuclear Bcl-6 protein expression was detected in 6 of 9 PCNSLs, 
      including those with genomic alterations of the encoding locus. Gains of 18q21 
      represented the most frequent imbalances present in more than one third of all 
      cases. Interestingly, these gains included the MLT gene. Thus, this study 
      provides the first evidence for recurrent chromosomal translocations in PCNSLs. 
      While they share similarities with extracerebral DLBCL with respect to the 
      presence of IGH translocations, they appear to differ in the usage of 
      translocation partner genes, which remain to be identified.
FAU - Montesinos-Rongen, Manuel
AU  - Montesinos-Rongen M
AD  - Department of Neuropathology, University of Cologne, Germany.
FAU - Zuhlke-Jenisch, Reina
AU  - Zuhlke-Jenisch R
FAU - Gesk, Stefan
AU  - Gesk S
FAU - Martin-Subero, Jose Ignacio
AU  - Martin-Subero JI
FAU - Schaller, Carlo
AU  - Schaller C
FAU - Van Roost, Dirk
AU  - Van Roost D
FAU - Wiestler, Otmar D
AU  - Wiestler OD
FAU - Deckert, Martina
AU  - Deckert M
FAU - Siebert, Reiner
AU  - Siebert R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Neuropathol Exp Neurol
JT  - Journal of neuropathology and experimental neurology
JID - 2985192R
SB  - IM
MH  - Adult
MH  - Aged
MH  - Brain Neoplasms/*genetics/pathology
MH  - *Chromosome Aberrations
MH  - *Chromosome Mapping
MH  - Cytogenetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphoma, B-Cell/*genetics/pathology
MH  - Middle Aged
EDAT- 2002/10/22 04:00
MHDA- 2002/11/26 04:00
CRDT- 2002/10/22 04:00
PHST- 2002/10/22 04:00 [pubmed]
PHST- 2002/11/26 04:00 [medline]
PHST- 2002/10/22 04:00 [entrez]
AID - 10.1093/jnen/61.10.926 [doi]
PST - ppublish
SO  - J Neuropathol Exp Neurol. 2002 Oct;61(10):926-33. doi: 10.1093/jnen/61.10.926.