PMID- 12388287
OWN - NLM
STAT- MEDLINE
DCOM- 20030117
LR  - 20200930
IS  - 0363-6135 (Print)
IS  - 0363-6135 (Linking)
VI  - 284
IP  - 1
DP  - 2003 Jan
TI  - Role of MMPs and plasminogen activators in angiogenesis after transmyocardial 
      laser revascularization in dogs.
PG  - H23-30
AB  - We examined the role of matrix metalloproteinases (MMPs), tissue inhibitors of 
      MMP (TIMPs), and plasminogen activator (PA) in transmyocardial laser 
      revascularization (TMLR)-induced angiogenesis. TMLR was accomplished with a 
      carbon dioxide laser in seven dogs whose left anterior descending coronary artery 
      (LAD) was ligated. Seven control dogs underwent only LAD ligation, and four dogs 
      underwent a sham operation, consisting only of a left thoracotomy. Two weeks 
      later, transmural myocardial samples were harvested from the distributions of the 
      LAD and the left circumflex artery for substrate zymography, immunohistochemical 
      staining, and in situ zymography. MMP-1, MMP-2, TIMP-1, TIMP-2, and 
      urokinase-type PA levels in the distribution of the LAD were higher in the laser 
      group than in the control or sham group. Counts of von Willebrand factor-positive 
      microvessels and smooth muscle alpha-actin-positive arterioles demonstrated that 
      the angiogenesis and ateriogenesis was promoted in the laser group and correlated 
      directly with the number of MMP-stained microvessels. We conclude that TMLR 
      induces the expression of MMPs, TIMPs, and urokinase-type PA and that these 
      proteinases play an important role in angiogenesis after TMLR.
FAU - Li, Wei
AU  - Li W
AD  - Second Department of Surgery, Fukui Medical University, Japan.
FAU - Tanaka, Kuniyoshi
AU  - Tanaka K
FAU - Chiba, Yukio
AU  - Chiba Y
FAU - Kimura, Tetsuya
AU  - Kimura T
FAU - Morioka, Kouichi
AU  - Morioka K
FAU - Uesaka, Takahiko
AU  - Uesaka T
FAU - Ihaya, Akio
AU  - Ihaya A
FAU - Sasaki, Masato
AU  - Sasaki M
FAU - Tsuda, Takeshi
AU  - Tsuda T
FAU - Yamada, Narihisa
AU  - Yamada N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20020905
PL  - United States
TA  - Am J Physiol Heart Circ Physiol
JT  - American journal of physiology. Heart and circulatory physiology
JID - 100901228
RN  - 0 (RNA, Messenger)
RN  - 0 (Tissue Inhibitor of Metalloproteinases)
RN  - EC 3.4.21.- (Plasminogen Activators)
RN  - EC 3.4.24.- (Gelatinases)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
SB  - IM
MH  - Animals
MH  - Dogs
MH  - Endocardium/pathology
MH  - Gelatinases/metabolism
MH  - Immunohistochemistry
MH  - *Laser Therapy
MH  - Matrix Metalloproteinases/*physiology
MH  - *Myocardial Revascularization
MH  - Neovascularization, Physiologic/*physiology
MH  - Pericardium/pathology
MH  - Plasminogen Activators/*physiology
MH  - Postoperative Period
MH  - RNA, Messenger/metabolism
MH  - Tissue Inhibitor of Metalloproteinases/genetics/metabolism
EDAT- 2002/10/22 04:00
MHDA- 2003/01/18 04:00
CRDT- 2002/10/22 04:00
PHST- 2002/10/22 04:00 [pubmed]
PHST- 2003/01/18 04:00 [medline]
PHST- 2002/10/22 04:00 [entrez]
AID - 00240.2002 [pii]
AID - 10.1152/ajpheart.00240.2002 [doi]
PST - ppublish
SO  - Am J Physiol Heart Circ Physiol. 2003 Jan;284(1):H23-30. doi: 
      10.1152/ajpheart.00240.2002. Epub 2002 Sep 5.