PMID- 12388334
OWN - NLM
STAT- MEDLINE
DCOM- 20030113
LR  - 20200930
IS  - 1040-0605 (Print)
IS  - 1040-0605 (Linking)
VI  - 284
IP  - 1
DP  - 2003 Jan
TI  - Surfactant protein A modulates the differentiation of murine bone marrow-derived 
      dendritic cells.
PG  - L232-41
AB  - Surfactant protein A (SP-A) is an innate immune molecule that regulates pathogen 
      clearance and lung inflammation. SP-A modulates innate immune functions such as 
      phagocytosis, cytokine production, and chemotaxis; however, little is known about 
      regulation of adaptive immunity by SP-A. Dendritic cells (DCs) are the most 
      potent antigen-presenting cell with the unique capacity to activate naive T cells 
      and initiate adaptive immunity. The goal of this study was to test the hypothesis 
      that SP-A regulates the differentiation of immature DCs into potent T cell 
      stimulators. The data show that incubation of immature DCs for 24 h with SP-A 
      inhibits basal- and LPS-mediated expression of major histocompatibility complex 
      class II and CD86. Stimulation of immature DCs by SP-A also inhibits the 
      allostimulation of T cells, enhances dextran endocytosis, and alters DC 
      chemotaxis toward RANTES and secondary lymphoid tissue chemokine. The effects on 
      DC phenotype and function are similar for the structurally homologous C1q, but 
      not for SP-D. These studies demonstrate that SP-A participates in the adaptive 
      immune response by modulating important immune functions of DCs.
FAU - Brinker, Karen G
AU  - Brinker KG
AD  - Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, 
      USA.
FAU - Garner, Hollie
AU  - Garner H
FAU - Wright, Jo Rae
AU  - Wright JR
LA  - eng
GR  - HL 68072/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20020913
PL  - United States
TA  - Am J Physiol Lung Cell Mol Physiol
JT  - American journal of physiology. Lung cellular and molecular physiology
JID - 100901229
RN  - 0 (Biomarkers)
RN  - 0 (Pulmonary Surfactant-Associated Protein A)
RN  - 80295-33-6 (Complement C1q)
SB  - IM
MH  - Animals
MH  - Biomarkers/analysis
MH  - Bone Marrow Cells/*cytology
MH  - Cell Differentiation/drug effects
MH  - Cells, Cultured
MH  - Cellular Senescence/physiology
MH  - Chemotaxis/drug effects
MH  - Complement C1q/pharmacology
MH  - Dendritic Cells/*cytology/drug effects/physiology
MH  - Endocytosis/drug effects
MH  - Female
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Phenotype
MH  - Pulmonary Surfactant-Associated Protein A/*pharmacology
EDAT- 2002/10/22 04:00
MHDA- 2003/01/14 04:00
CRDT- 2002/10/22 04:00
PHST- 2002/10/22 04:00 [pubmed]
PHST- 2003/01/14 04:00 [medline]
PHST- 2002/10/22 04:00 [entrez]
AID - 00187.2002 [pii]
AID - 10.1152/ajplung.00187.2002 [doi]
PST - ppublish
SO  - Am J Physiol Lung Cell Mol Physiol. 2003 Jan;284(1):L232-41. doi: 
      10.1152/ajplung.00187.2002. Epub 2002 Sep 13.