PMID- 12390882
OWN - NLM
STAT- MEDLINE
DCOM- 20030521
LR  - 20190513
IS  - 0006-3363 (Print)
IS  - 0006-3363 (Linking)
VI  - 67
IP  - 5
DP  - 2002 Nov
TI  - Determination of cell type specificity and estrous cycle dependency of monocyte 
      chemoattractant protein-1 expression in corpora lutea of normally cycling rats in 
      relation to apoptosis and monocyte/macrophage accumulation.
PG  - 1502-8
AB  - In regressive corpora lutea, apoptosis of luteal cells, expression of monocyte 
      chemoattractant protein-1 (MCP-1), and accumulation of monocytes/macrophages 
      occur. However, whether these three events are correlated and what cell type 
      expresses MCP-1 have yet to be determined. To clarify these issues, we performed 
      histochemical examinations to determine the localization and the numbers of MCP-1 
      mRNA-containing cells, apoptotic cells, and monocytes/macrophages in corpora 
      lutea of normally cycling rats. We found that the Mcp-1 gene is expressed in 
      nonapoptotic steroidogenic luteal cells. Corpora lutea that contained MCP-1 
      mRNA-expressing cells increased in number at estrus together with those 
      containing apoptotic luteal cells. When individual corpora lutea at estrus were 
      analyzed, those with many MCP-1-expressing cells contained few apoptotic cells, 
      and vice versa. These results collectively suggest the following pathway for 
      apoptosis- and MCP-1-dependent regression of the corpus luteum: 1) luteal cells 
      are induced to undergo apoptosis at estrus, and the activation of Mcp-1 gene 
      expression follows in nonapoptotic luteal cells; 2) monocytes/macrophages are 
      chemoattracted by MCP-1 toward corpora lutea containing apoptotic luteal cells; 
      and 3) monocytes/macrophages invade corpora lutea and eliminate apoptotic luteal 
      cells by phagocytosis.
FAU - Nagaosa, Kaz
AU  - Nagaosa K
AD  - Graduate School of Natural Science and Technology. Graduate School of Medical 
      Science, Kanazawa University, Takara-machi, Kanazawa, Ishikawa 920-0934, Japan.
FAU - Shiratsuchi, Akiko
AU  - Shiratsuchi A
FAU - Nakanishi, Yoshinobu
AU  - Nakanishi Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Apoptosis/*physiology
MH  - Chemokine CCL2/*genetics
MH  - Corpus Luteum/cytology/*physiology
MH  - Estrous Cycle/*physiology
MH  - Female
MH  - Gene Expression
MH  - Macrophages/*physiology
MH  - Monocytes/*physiology
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Inbred Strains
EDAT- 2002/10/23 04:00
MHDA- 2003/05/22 05:00
CRDT- 2002/10/23 04:00
PHST- 2002/10/23 04:00 [pubmed]
PHST- 2003/05/22 05:00 [medline]
PHST- 2002/10/23 04:00 [entrez]
AID - 10.1095/biolreprod.102.005009 [doi]
PST - ppublish
SO  - Biol Reprod. 2002 Nov;67(5):1502-8. doi: 10.1095/biolreprod.102.005009.