PMID- 12391196
OWN - NLM
STAT- MEDLINE
DCOM- 20021210
LR  - 20220311
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 169
IP  - 9
DP  - 2002 Nov 1
TI  - Monocyte chemoattractant protein-1 selectively inhibits the acquisition of CD40 
      ligand-dependent IL-12-producing capacity of monocyte-derived dendritic cells and 
      modulates Th1 immune response.
PG  - 4861-6
AB  - Accumulating evidence indicates that monocyte chemoattractant protein-1 (MCP-1), 
      a CC chemokine, also displays immunoregulatory functions and may be involved in 
      Th subset differentiation. In this study, we examined the effects of MCP-1 on the 
      cytokine-driven differentiation of monocytes into dendritic cells (DCs), the most 
      potent APCs for naive T cells. We found that DCs generated in the presence of 
      MCP-1 displayed a markedly reduced production of IL-12 in response to CD40 ligand 
      but not in response to Staphylococcus aureus stimulation in the presence or 
      absence of IFN-gamma. The production of IL-10, a potent endogenous IL-12 
      inhibitor, was not affected by MCP-1. Whereas the inhibitory activity of MCP-1 on 
      IL-12 production by monocytes was sensitive to pertussis toxin, its effects on DC 
      differentiation were pertussis toxin resistant. MCP-1 did not affect the surface 
      phenotype and T cell-stimulating activity of DCs, but most interestingly, naive T 
      cells stimulated with MCP-1-primed DCs produced much less IFN-gamma but the same 
      levels of IL-13. Taken together, our results indicated that MCP-1 modulates the 
      differentiation of monocytes into DCs and may thereby inhibit Th1 cell 
      development.
FAU - Omata, Nemuko
AU  - Omata N
AD  - Department of Pediatrics, Faculty of Medicine, Fukui Medical University, Japan.
FAU - Yasutomi, Motoko
AU  - Yasutomi M
FAU - Yamada, Akiko
AU  - Yamada A
FAU - Iwasaki, Hiromichi
AU  - Iwasaki H
FAU - Mayumi, Mitsufumi
AU  - Mayumi M
FAU - Ohshima, Yusei
AU  - Ohshima Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (CD40 Antigens)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL4)
RN  - 0 (Immunosuppressive Agents)
RN  - 0 (Macrophage Inflammatory Proteins)
RN  - 147205-72-9 (CD40 Ligand)
RN  - 187348-17-0 (Interleukin-12)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 2.4.2.31 (Pertussis Toxin)
SB  - IM
MH  - Adjuvants, Immunologic/*pharmacology
MH  - CD40 Antigens/physiology
MH  - CD40 Ligand/*physiology
MH  - Cell Differentiation/immunology
MH  - Cells, Cultured
MH  - Chemokine CCL2/*pharmacology
MH  - Chemokine CCL4
MH  - Dendritic Cells/cytology/*immunology/metabolism
MH  - Dose-Response Relationship, Immunologic
MH  - Down-Regulation/immunology
MH  - Humans
MH  - Immunosuppressive Agents/*pharmacology
MH  - Interferon-gamma/biosynthesis
MH  - Interleukin-12/*antagonists & inhibitors/*biosynthesis
MH  - Interphase/immunology
MH  - Kinetics
MH  - Macrophage Inflammatory Proteins/pharmacology
MH  - Monocytes/cytology/*immunology/metabolism
MH  - Pertussis Toxin/pharmacology
MH  - Th1 Cells/*immunology/metabolism
EDAT- 2002/10/23 04:00
MHDA- 2002/12/11 04:00
CRDT- 2002/10/23 04:00
PHST- 2002/10/23 04:00 [pubmed]
PHST- 2002/12/11 04:00 [medline]
PHST- 2002/10/23 04:00 [entrez]
AID - 10.4049/jimmunol.169.9.4861 [doi]
PST - ppublish
SO  - J Immunol. 2002 Nov 1;169(9):4861-6. doi: 10.4049/jimmunol.169.9.4861.