PMID- 12392645
OWN - NLM
STAT- MEDLINE
DCOM- 20030702
LR  - 20061115
IS  - 0014-2565 (Print)
IS  - 0014-2565 (Linking)
VI  - 202
IP  - 11
DP  - 2002 Nov
TI  - [Lack of human herpesvirus type 6 DNA in CSF by nested PCR among patients with 
      multiple sclerosis].
PG  - 588-91
AB  - BACKGROUND: The etiology of multiple sclerosis (MS) is currently unknown. 
      Different viruses have tentatively been involved as causative agents of MS that 
      would trigger an autoimmune response leading to demyelination plaques. There is 
      controversy regarding the role that the human herpesvirus 6 (HHV-6) might play in 
      this condition, and high antibody titers have been detected to HHV-6. HHV-6 DNA 
      has also been detected by PCR both in blood and cerebrospinal fluid by means of 
      the Polymerase Chain Reaction (PCR). Immunohistochemistry studies were performed 
      with histologic specimens from the white matters of patientes with MS. All this 
      has led some authors to incriminate this virus as the triggering etiologic agent 
      of this disease. PATIENTS AND METHODS: CSF specimens from 23 patients with 
      Relapsing-Remitting MS were studied. The CSF specimens from 23 patients 
      undergoing rachianesthesia were used as controls, and none of them had neurologic 
      disorders. A nested PCR was performed in the collected specimens to detect 
      specific DNA sequences of HHV-6. RESULTS: No DNA sequences of HHV-6, EBV, VZV, 
      CMV and HSV were detected in the tested specimens. CONCLUSIONS: No HHV-6 DNA 
      sequences were detected from CSF specimens of patients with MS. Further 
      investigations on the association between HHV-6 and MS should be performed to 
      elucidate the role of HHV-6 in the pathogenesis of this disease.
FAU - Rodriguez Carnero, S
AU  - Rodriguez Carnero S
AD  - Unidad de Enfermedades Infecciosas-Servicio de Medicina Interna. Complexo 
      Hospitalario Xeral-Cies. Vigo. Pontevedra. Spain.
FAU - Martinez-Vazquez, C
AU  - Martinez-Vazquez C
FAU - Potel Alvarellos, C
AU  - Potel Alvarellos C
FAU - Alvarez Fernandez, M
AU  - Alvarez Fernandez M
FAU - Prieto Gonzalez, J M
AU  - Prieto Gonzalez JM
FAU - Noya Garcia, M
AU  - Noya Garcia M
FAU - de la Fuente Aguado, J
AU  - de la Fuente Aguado J
FAU - Sopena Arguelles, B
AU  - Sopena Arguelles B
LA  - spa
PT  - English Abstract
PT  - Journal Article
TT  - Ausencia de ADN de herpesvirus humano 6 en LCR, mediante tecnica de PCR anidada, 
      en pacientes con esclerosis multiple.
PL  - Spain
TA  - Rev Clin Esp
JT  - Revista clinica espanola
JID - 8608576
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adult
MH  - DNA, Viral/*cerebrospinal fluid
MH  - Female
MH  - Herpesvirus 6, Human/*genetics
MH  - Humans
MH  - Male
MH  - Multiple Sclerosis/*virology
MH  - Polymerase Chain Reaction
EDAT- 2002/10/24 04:00
MHDA- 2003/07/03 05:00
CRDT- 2002/10/24 04:00
PHST- 2002/10/24 04:00 [pubmed]
PHST- 2003/07/03 05:00 [medline]
PHST- 2002/10/24 04:00 [entrez]
AID - 13037921 [pii]
PST - ppublish
SO  - Rev Clin Esp. 2002 Nov;202(11):588-91.