PMID- 12392849
OWN - NLM
STAT- MEDLINE
DCOM- 20030411
LR  - 20190906
IS  - 0198-8859 (Print)
IS  - 0198-8859 (Linking)
VI  - 63
IP  - 11
DP  - 2002 Nov
TI  - Analysis of HLA-G expression in breast cancer tissues.
PG  - 969-76
AB  - Among the different mechanisms by which cancer can elude the immune system, 
      alterations in the expression of human leukocyte antigen (HLA) class I molecules 
      on tumor cells may play a crucial role by impairing the HLA molecules interaction 
      with T and natural killer (NK) cells specific receptors. More recently, aberrant 
      expression of HLA-G has been described in different tumor tissues in addition to 
      HLA class I downregulation. The HLA-G molecule is a nonclassical HLA class I 
      antigen selectively expressed by trophoblast and thymic epithelial cells. Several 
      studies reported that the HLA-G function might represent an additional mechanism 
      of tumor immune escape, mainly inhibiting NK and cytotoxic T-cell activity. Here 
      we report the analysis of HLA-G expression both at RNA level by reverse 
      transcriptase-polymerase chain reaction and at protein level by Western blot and 
      immunohistochemistry in 25 breast cancer patient tissues. The aim of this study 
      was to elucidate the HLA-G gene expression pattern in breast tumor tissues and 
      correlate it with HLA class I alterations. Our results demonstrated that HLA-G 
      molecules expression was never found even in a group of patients revealing HLA 
      class I total loss, and that HLA-G is not expressed in breast cancer tissue with 
      a low-tumor grade (G1-G2) and minimal stromal contamination.
FAU - Palmisano, Giulio Lelio
AU  - Palmisano GL
AD  - Laboratory of Molecular Morphogenesis, National Cancer Research Institute, 
      Genova, Italy. palmisano@vega.cba.unige.it
FAU - Pistillo, Maria Pia
AU  - Pistillo MP
FAU - Fardin, Paolo
AU  - Fardin P
FAU - Capanni, Paolo
AU  - Capanni P
FAU - Nicolo, Guido
AU  - Nicolo G
FAU - Salvi, Sandra
AU  - Salvi S
FAU - Spina, Bruno
AU  - Spina B
FAU - Pasciucco, Gennaro
AU  - Pasciucco G
FAU - Ferrara, Giovanni Battista
AU  - Ferrara GB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-G Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
MH  - Blotting, Western
MH  - Breast Neoplasms/*immunology
MH  - Female
MH  - Genes, MHC Class I
MH  - HLA Antigens/*analysis/genetics/physiology
MH  - HLA-G Antigens
MH  - Histocompatibility Antigens Class I/*analysis/genetics/physiology
MH  - Humans
MH  - Immunohistochemistry
MH  - K562 Cells
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tumor Cells, Cultured
EDAT- 2002/10/24 04:00
MHDA- 2003/04/12 05:00
CRDT- 2002/10/24 04:00
PHST- 2002/10/24 04:00 [pubmed]
PHST- 2003/04/12 05:00 [medline]
PHST- 2002/10/24 04:00 [entrez]
AID - S0198885902006420 [pii]
AID - 10.1016/s0198-8859(02)00642-0 [doi]
PST - ppublish
SO  - Hum Immunol. 2002 Nov;63(11):969-76. doi: 10.1016/s0198-8859(02)00642-0.