PMID- 12392951
OWN - NLM
STAT- MEDLINE
DCOM- 20030618
LR  - 20191210
IS  - 0956-5663 (Print)
IS  - 0956-5663 (Linking)
VI  - 17
IP  - 11-12
DP  - 2002 Dec
TI  - Disposable tyrosinase-peroxidase bi-enzyme sensor for amperometric detection of 
      phenols.
PG  - 1015-23
AB  - A new disposable amperometric bi-enzyme sensor system for detecting phenols has 
      been developed. The phenol sensor developed uses horseradish peroxidase modified 
      screen-printed carbon electrodes (HRP-SPCEs) coupled with immobilized tyrosinase 
      prepared using poly(carbamoylsulfonate) (PCS) hydrogels or a poly(vinyl alcohol) 
      bearing styrylpyridinium groups (PVA-SbQ) matrix. Optimization of the 
      experimental parameters has been performed with regard to buffer composition, pH, 
      operating potential and storage stability. A co-operative reaction involving 
      tyrosinase and HRP occurs at a potential of -50 mV versus Ag/AgCl without the 
      requirement for addition of extraneous H(2)O(2), thus, resulting in a very simple 
      and efficient system. Comparison of the electrode responses with the 
      4-aminoantipyrine standard method for phenol sample analysis indicated the 
      feasibility of the disposable sensor system for sensitive "in-field" 
      determination of phenols. The most sensitive system was the tyrosinase 
      immobilized HRP-SPCE using PCS, which displayed detection limits for phenolic 
      compounds in the lower nanomolar range e.g. 2.5 nM phenol, 10 nM catechol and 5 
      nM p-cresol.
FAU - Chang, Seung Cheol
AU  - Chang SC
AD  - School of Clinical and Laboratory Sciences, The Medical School, University of 
      Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, UK.
FAU - Rawson, Keith
AU  - Rawson K
FAU - McNeil, Calum J
AU  - McNeil CJ
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Enzymes, Immobilized)
RN  - 0 (Multienzyme Complexes)
RN  - 0 (Phenols)
RN  - 0 (Water Pollutants, Chemical)
RN  - 7440-44-0 (Carbon)
RN  - EC 1.11.1.- (Horseradish Peroxidase)
RN  - EC 1.14.18.1 (Monophenol Monooxygenase)
SB  - IM
MH  - Biosensing Techniques/*instrumentation/methods
MH  - Calibration
MH  - Carbon
MH  - Disposable Equipment
MH  - Electrochemistry/*instrumentation/methods
MH  - Electrodes
MH  - Enzymes, Immobilized/chemistry
MH  - Equipment Design
MH  - Equipment Failure Analysis
MH  - Feasibility Studies
MH  - Horseradish Peroxidase/chemistry
MH  - Hydrogen-Ion Concentration
MH  - Monophenol Monooxygenase/chemistry
MH  - Multienzyme Complexes/chemistry
MH  - Oxidation-Reduction
MH  - Phenols/*analysis/chemistry
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Water Pollutants, Chemical/analysis
EDAT- 2002/10/24 04:00
MHDA- 2003/06/19 05:00
CRDT- 2002/10/24 04:00
PHST- 2002/10/24 04:00 [pubmed]
PHST- 2003/06/19 05:00 [medline]
PHST- 2002/10/24 04:00 [entrez]
AID - S0956566302000945 [pii]
AID - 10.1016/s0956-5663(02)00094-5 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2002 Dec;17(11-12):1015-23. doi: 
      10.1016/s0956-5663(02)00094-5.