PMID- 12393099
OWN - NLM
STAT- MEDLINE
DCOM- 20021216
LR  - 20190513
IS  - 0008-6363 (Print)
IS  - 0008-6363 (Linking)
VI  - 56
IP  - 2
DP  - 2002 Nov
TI  - MCP-1 induces activation of MAP-kinases ERK, JNK and p38 MAPK in human 
      endothelial cells.
PG  - 284-92
AB  - Activation of vascular endothelial cells (ECs) plays an important pathogenic role 
      in the development of atherosclerosis. Monocyte chemoattractant protein-1 (MCP-1) 
      is a potent chemoattractant of monocytes. Besides induction of monocyte 
      recruitment, it has been suggested that MCP-1 can also affect the cellular 
      responses of ECs. We investigated whether MCP-1 activated the three major mitogen 
      activated protein (MAP)-kinases extracellular signal-regulated kinase (ERK), 
      c-Jun amino terminal kinase (JNK) and p38 MAPK. Stimulation of ECs with MCP-1 
      induced a time- and concentration-dependent activation of all three MAP-kinases, 
      concentrations as low as 0.1 ng/ml were sufficient for this mechanism. MCP-1 also 
      induced secretion of matrix metalloproteinase (MMP)-2 which along with ERK 
      activation was inhibited by PD098059. The results demonstrate that MCP-1 can lead 
      to direct activation of MAP kinases together with induction of MMP2 in ECs. Our 
      data thus propose a new mechanism for the proatherogenic effect of MCP-1.
FAU - Werle, Martina
AU  - Werle M
AD  - Innere Medizin III, Universitat Heidelberg, Bergheimer Strasse, 58 69115, 
      Heidelberg, Germany.
FAU - Schmal, Ulrike
AU  - Schmal U
FAU - Hanna, Katharina
AU  - Hanna K
FAU - Kreuzer, Jorg
AU  - Kreuzer J
LA  - eng
PT  - Journal Article
PL  - England
TA  - Cardiovasc Res
JT  - Cardiovascular research
JID - 0077427
RN  - 0 (CCR2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, CCR2)
RN  - 0 (Receptors, Chemokine)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Cell Culture Techniques
MH  - Chemokine CCL2/*pharmacology
MH  - Endothelium, Vascular/*drug effects/*enzymology
MH  - Gene Expression
MH  - Humans
MH  - JNK Mitogen-Activated Protein Kinases
MH  - Mitogen-Activated Protein Kinases/*metabolism
MH  - Phosphorylation
MH  - RNA, Messenger/genetics
MH  - Receptors, CCR2
MH  - Receptors, Chemokine/genetics/metabolism
MH  - Signal Transduction
MH  - p38 Mitogen-Activated Protein Kinases
EDAT- 2002/10/24 04:00
MHDA- 2002/12/18 04:00
CRDT- 2002/10/24 04:00
PHST- 2002/10/24 04:00 [pubmed]
PHST- 2002/12/18 04:00 [medline]
PHST- 2002/10/24 04:00 [entrez]
AID - S0008636302006004 [pii]
AID - 10.1016/s0008-6363(02)00600-4 [doi]
PST - ppublish
SO  - Cardiovasc Res. 2002 Nov;56(2):284-92. doi: 10.1016/s0008-6363(02)00600-4.