PMID- 12393279
OWN - NLM
STAT- MEDLINE
DCOM- 20021114
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 137
IP  - 2
DP  - 2002 Sep
TI  - Interphase fluorescence in situ hybridization detection of chromosome 17 and 17q 
      region gains in neuroblastoma: are they secondary events?
PG  - 95-101
AB  - Gains of chromosome 17 and 17q region are the most frequent chromosomal 
      abnormalities in neuroblastoma and have been associated with established 
      prognostic indicators. Interphase fluorescence in situ hybridization (FISH) was 
      used to define the status of chromosome 17 in near-triploid (3n) and 
      near-diploid/tetraploid (2n/4n) primary tumors. Gains of chromosome 17 and 17q 
      were detected in 22 and 26 tumors, respectively, in which the ploidy status was 
      determined mainly by the copy number of chromosome 1. Four different types of 
      gains were detected: gain of whole chromosome 17 (+17) and three partial gains 
      (17q11.2 approximately qter, 17q21.1 approximately qter, and 17q21.3 
      approximately qter). The 17q11.2 approximately qter gains were found in both the 
      2n/4n and the 3n tumors. Gains of 17q21.1 approximately qter and 17q21.3 
      approximately qter were found only in the 2n/4n group, and the latter was 
      involved always as a der(22)t(17;22)(q21;q13). A high association was found 
      between chromosome 17 gains and 3n ploidy: +17 was detected in 93% of the 3n 
      group and was not observed in the 2n/4n group. The +17 clone or clones were 
      always present in combination with a clone with normal copies of chromosome 17 
      and, in the majority, with a +17q11.2 approximately qter clone. We conclude that 
      interphase FISH is a sensitive method for detecting whole and partial chromosome 
      17 gains in neuroblastoma and can demonstrate the simultaneous presence of 
      several clones with different status of chromosome 17 in 3n neuroblastomas. We 
      suggest that chromosome 17 and 17q gains are not a primary event in the 
      development of neuroblastoma.
FAU - Trakhtenbrot, Luba
AU  - Trakhtenbrot L
AD  - Department of Pediatric Hemato-Oncology and Institute of Hematology, The Chaim 
      Sheba Medical Center, Tel Hashomer, 52621 Israel. lubatr@yahoo.com
FAU - Cohen, Ninette
AU  - Cohen N
FAU - Betts, David R
AU  - Betts DR
FAU - Niggli, Felix K
AU  - Niggli FK
FAU - Amariglio, Ninette
AU  - Amariglio N
FAU - Brok-Simoni, Frida
AU  - Brok-Simoni F
FAU - Rechavi, Gideon
AU  - Rechavi G
FAU - Meitar, Dafna
AU  - Meitar D
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 1/genetics
MH  - Chromosomes, Human, Pair 17/*genetics
MH  - Cytogenetic Analysis
MH  - Genes, myc/genetics
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Infant
MH  - Infant, Newborn
MH  - Interphase
MH  - Neoplasm Staging
MH  - Neuroblastoma/*genetics/pathology
MH  - Ploidies
MH  - Spectral Karyotyping
MH  - Tumor Cells, Cultured
EDAT- 2002/10/24 04:00
MHDA- 2002/11/26 04:00
CRDT- 2002/10/24 04:00
PHST- 2002/10/24 04:00 [pubmed]
PHST- 2002/11/26 04:00 [medline]
PHST- 2002/10/24 04:00 [entrez]
AID - S0165460802005538 [pii]
AID - 10.1016/s0165-4608(02)00553-8 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2002 Sep;137(2):95-101. doi: 
      10.1016/s0165-4608(02)00553-8.