PMID- 12393283 OWN - NLM STAT- MEDLINE DCOM- 20021114 LR - 20190816 IS - 0165-4608 (Print) IS - 0165-4608 (Linking) VI - 137 IP - 2 DP - 2002 Sep TI - Molecular cytogenetic analysis of the monoblastic cell line U937. karyotype clarification by G-banding, whole chromosome painting, microdissection and reverse painting, and comparative genomic hybridization. PG - 124-32 AB - Previous reports on the analysis of the human monoblastic cell line U937 had described several sublines containing unidentified rearrangements and marker chromosomes. In order to determine the true nature of the rearrangements, conventional banding analysis was carried out with various combinations of molecular cytogenetic techniques: comparative genomic hybridization, fluorescence in situ hybridization (FISH) with whole chromosome painting probes, and microdissection and reverse painting FISH. The origins of the marker chromosomes were identified and the composite karyotype is described. FAU - Lee, Ji-Yun AU - Lee JY AD - Department of Medical Genetics, College of Medicine, Hanyang University, Seoul 133-791, South Korea. FAU - Lee, Chul-Hoon AU - Lee CH FAU - Shim, Sung-Han AU - Shim SH FAU - Seo, Han-Kyu AU - Seo HK FAU - Kyhm, Jee-Hong AU - Kyhm JH FAU - Cho, Sechin AU - Cho S FAU - Cho, Youl-Hee AU - Cho YH LA - eng PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Cancer Genet Cytogenet JT - Cancer genetics and cytogenetics JID - 7909240 SB - IM MH - Chromosome Banding/*methods MH - Chromosome Painting/*methods MH - Chromosomes, Human/genetics MH - Dissection MH - Humans MH - In Situ Hybridization, Fluorescence MH - Karyotyping MH - Nucleic Acid Hybridization/*methods MH - U937 Cells EDAT- 2002/10/24 04:00 MHDA- 2002/11/26 04:00 CRDT- 2002/10/24 04:00 PHST- 2002/10/24 04:00 [pubmed] PHST- 2002/11/26 04:00 [medline] PHST- 2002/10/24 04:00 [entrez] AID - S0165460802005654 [pii] AID - 10.1016/s0165-4608(02)00565-4 [doi] PST - ppublish SO - Cancer Genet Cytogenet. 2002 Sep;137(2):124-32. doi: 10.1016/s0165-4608(02)00565-4.