PMID- 12393409
OWN - NLM
STAT- MEDLINE
DCOM- 20030408
LR  - 20210206
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 101
IP  - 2
DP  - 2003 Jan 15
TI  - Frequent occurrence of BCL6 rearrangements in nodular lymphocyte predominance 
      Hodgkin lymphoma but not in classical Hodgkin lymphoma.
PG  - 706-10
AB  - We studied the genomic status of BCL6 in 23 cases of nodular lymphocyte 
      predominance Hodgkin lymphoma (NLPHL) and 40 cases of classical Hodgkin lymphoma 
      (cHL), using dual-color interphase fluorescence in situ hybridization (FISH). The 
      BCL6 rearrangement was identified in 48% of NLPHL cases and was not detected in 
      cHL cases. As a confirmation, sequential or simultaneous immunohistochemistry 
      (IHC) and FISH using CD20 or BCL6 antibodies and BCL6 DNA probes was performed in 
      8 NLPHL cases. The BCL6-associated translocations, t(3;22)(q27;q11), 
      t(3;7)(q27;p12), and the most probable t(3;9)(q27;p13), were identified in 3 
      cases. A consistent expression of BCL6 protein in popcorn cells with the highest 
      number of intensely stained cells in cases with a genomic BCL6 rearrangement was 
      shown by IHC. These findings support the hypothesis of a germinal center B 
      cell-derived origin of NLPHL, indicate a significant role of BCL6 in the 
      pathogenesis of NLPHL, and provide further evidence of the genetic diversity 
      underlying the pathogenesis of NLPHL and cHL.
FAU - Wlodarska, Iwona
AU  - Wlodarska I
AD  - Center for Human Genetics, Catholic University of Leuven, Belgium.
FAU - Nooyen, Peet
AU  - Nooyen P
FAU - Maes, Brigitte
AU  - Maes B
FAU - Martin-Subero, Jose I
AU  - Martin-Subero JI
FAU - Siebert, Reiner
AU  - Siebert R
FAU - Pauwels, Patrick
AU  - Pauwels P
FAU - De Wolf-Peeters, Chris
AU  - De Wolf-Peeters C
FAU - Hagemeijer, Anne
AU  - Hagemeijer A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20020912
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (DNA, Neoplasm)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins c-bcl-6)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - B-Lymphocytes/pathology
MH  - DNA, Neoplasm/analysis
MH  - DNA-Binding Proteins/analysis/*genetics
MH  - Diagnosis, Differential
MH  - Gene Rearrangement, T-Lymphocyte
MH  - Germinal Center/pathology
MH  - Hodgkin Disease/etiology/*genetics/*pathology
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Incidence
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/etiology/*genetics/*pathology
MH  - Molecular Diagnostic Techniques
MH  - Proto-Oncogene Proteins/analysis/*genetics
MH  - Proto-Oncogene Proteins c-bcl-6
MH  - Transcription Factors/analysis/*genetics
MH  - Translocation, Genetic
EDAT- 2002/10/24 04:00
MHDA- 2003/04/09 05:00
CRDT- 2002/10/24 04:00
PHST- 2002/10/24 04:00 [pubmed]
PHST- 2003/04/09 05:00 [medline]
PHST- 2002/10/24 04:00 [entrez]
AID - S0006-4971(20)44533-1 [pii]
AID - 10.1182/blood-2002-05-1592 [doi]
PST - ppublish
SO  - Blood. 2003 Jan 15;101(2):706-10. doi: 10.1182/blood-2002-05-1592. Epub 2002 Sep 
      12.