PMID- 12393481
OWN - NLM
STAT- MEDLINE
DCOM- 20030313
LR  - 20210206
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 101
IP  - 4
DP  - 2003 Feb 15
TI  - Dendritic cells overexpressing CD95 (Fas) ligand elicit vigorous allospecific 
      T-cell responses in vivo.
PG  - 1469-76
AB  - Dendritic cells (DCs) genetically engineered to overexpress CD95 (Fas) ligand 
      (CD95L-DC) were proposed as tools to induce peripheral tolerance to alloantigens. 
      Herein, we observed that CD95L-DC obtained after retroviral gene transfer in bone 
      marrow (BM) precursors derived from CD95-deficient (lpr/lpr) mice elicit much 
      stronger allospecific type 1 helper T-cell and cytotoxic T-cell activities than 
      control DCs upon injection in vivo, although they induce lower T-cell responses 
      in vitro. Indeed, a single injection of CD95L-DC prepared from C57BL/6 mice was 
      sufficient to prime bm13 recipients for acute rejection of C57BL/6 skin 
      allografts that were otherwise tolerated in the context of this single weak major 
      histocompatibility complex (MHC) class I incompatibility. Massive neutrophil 
      infiltrates depending on interleukin (IL)-1 signaling were observed at sites of 
      CD95L-DC injection. Experiments in IL-1 receptor-deficient mice or in animals 
      injected with depleting anti-Gr1 monoclonal antibody (mAb) established that 
      neutrophil recruitment is required for the development of vigorous T-cell 
      responses after injection of CD95L-DC in vivo.
FAU - Buonocore, Sofia
AU  - Buonocore S
AD  - Laboratory of Experimental Immunology, Universite Libre de Bruxelles, Belgium.
FAU - Paulart, Frederic
AU  - Paulart F
FAU - Le Moine, Alain
AU  - Le Moine A
FAU - Braun, Michel
AU  - Braun M
FAU - Salmon, Isabelle
AU  - Salmon I
FAU - Van Meirvenne, Sonja
AU  - Van Meirvenne S
FAU - Thielemans, Kris
AU  - Thielemans K
FAU - Goldman, Michel
AU  - Goldman M
FAU - Flamand, Veronique
AU  - Flamand V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20021017
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Interleukin-2)
RN  - 0 (Interleukin-5)
RN  - 0 (Isoantigens)
RN  - 0 (Receptors, Interleukin-1)
RN  - 0 (fas Receptor)
RN  - 207137-56-2 (Interleukin-4)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/pharmacology
MH  - Dendritic Cells/*immunology
MH  - Graft Rejection/immunology
MH  - Immune Tolerance
MH  - Interferon-gamma/biosynthesis
MH  - Interleukin-2/biosynthesis
MH  - Interleukin-4/biosynthesis
MH  - Interleukin-5/biosynthesis
MH  - Isoantigens/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Neutrophils/immunology
MH  - Receptors, Interleukin-1/deficiency
MH  - Retroviridae/genetics
MH  - Skin Transplantation
MH  - T-Lymphocytes/*immunology
MH  - T-Lymphocytes, Cytotoxic/immunology
MH  - T-Lymphocytes, Helper-Inducer/immunology
MH  - Transfection
MH  - fas Receptor/*genetics/immunology/physiology
EDAT- 2002/10/24 04:00
MHDA- 2003/03/14 04:00
CRDT- 2002/10/24 04:00
PHST- 2002/10/24 04:00 [pubmed]
PHST- 2003/03/14 04:00 [medline]
PHST- 2002/10/24 04:00 [entrez]
AID - S0006-4971(20)57413-2 [pii]
AID - 10.1182/blood-2002-07-2042 [doi]
PST - ppublish
SO  - Blood. 2003 Feb 15;101(4):1469-76. doi: 10.1182/blood-2002-07-2042. Epub 2002 Oct 
      17.