PMID- 12393628
OWN - NLM
STAT- MEDLINE
DCOM- 20030128
LR  - 20210206
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 100
IP  - 13
DP  - 2002 Dec 15
TI  - Characterization of human blood dendritic cell subsets.
PG  - 4512-20
AB  - Dendritic cells (DCs) are key antigen-presenting cells for stimulating immune 
      responses and they are now being investigated in clinical settings. Although 
      defined as lineage-negative (Lin(-)) HLA-DR(+) cells, significant heterogeneity 
      in these preparations is apparent, particularly in regard to the inclusion or 
      exclusion of CD14(+), CD16(+), and CD2(+) cells. This study used flow cytometry 
      and a panel of monoclonal antibodies (mAbs), including reagents from the 7th 
      Leukocyte Differentiation Antigen Workshop, to define the cellular composition of 
      2 standardized peripheral blood mononuclear cell (PBMCs)-derived Lin(-) HLA-DR(+) 
      preparations. Lin(-) cells were prepared from PBMCs by depletion with CD3, CD14, 
      CD19, CD11b, and either CD16 or CD56 mAbs. Analysis of the CD16-replete 
      preparations divided the Lin(-) HLA-DR(+) population into 5 nonoverlapping 
      subsets (mean +/- 1 SD): CD123 (mean = 18.3% +/- 9.7%), CD1b/c (18.6% +/- 7.6%), 
      CD16 (49.6% +/- 8.5%), BDCA-3 (2.7% +/- 1.4%), and CD34 (5.0% +/- 2.4%). The 5 
      subsets had distinct phenotypes when compared with each other, monocytes, and 
      monocyte-derived DCs (MoDCs). The CD85 family, C-type lectins, costimulatory 
      molecules, and differentiation/activation molecules were also expressed 
      differentially on the 5 Lin(-) HLA-DR(+) subsets, monocytes, and MoDCs. The poor 
      viability of CD123(+) DCs in vitro was confirmed, but the CD16(+) CD11c(+) DC 
      subset also survived poorly. Finally, the individual subsets used as stimulators 
      in allogeneic mixed leukocyte reactions were ranked by their allostimulatory 
      capacity as CD1b/c > CD16 > BDCA-3 > CD123 > CD34. These data provide an 
      opportunity to standardize the DC populations used for future molecular, 
      functional and possibly even therapeutic studies.
FAU - MacDonald, Kelli P A
AU  - MacDonald KP
AD  - Dendritic Cell Laboratory, Mater Medical Research Institute, Mater Misericordiae 
      Hospitals, South Brisbane, Australia.
FAU - Munster, David J
AU  - Munster DJ
FAU - Clark, Georgina J
AU  - Clark GJ
FAU - Dzionek, Andrzej
AU  - Dzionek A
FAU - Schmitz, Juergen
AU  - Schmitz J
FAU - Hart, Derek N J
AU  - Hart DN
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20020815
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Surface)
RN  - 0 (Lectins)
SB  - IM
MH  - Adult
MH  - Antibodies, Monoclonal/immunology
MH  - Antigen Presentation
MH  - Antigens, CD/analysis
MH  - Antigens, Surface/analysis
MH  - Blood Cells/classification
MH  - Cell Separation
MH  - Cell Survival
MH  - Cells, Cultured/cytology
MH  - Dendritic Cells/chemistry/*classification/immunology
MH  - Flow Cytometry
MH  - Gene Expression Profiling
MH  - Humans
MH  - Immunophenotyping
MH  - Lectins/metabolism
MH  - Lymphocyte Culture Test, Mixed
MH  - Monocytes/cytology
MH  - Reference Standards
EDAT- 2002/10/24 04:00
MHDA- 2003/01/29 04:00
CRDT- 2002/10/24 04:00
PHST- 2002/10/24 04:00 [pubmed]
PHST- 2003/01/29 04:00 [medline]
PHST- 2002/10/24 04:00 [entrez]
AID - S0006-4971(20)53658-6 [pii]
AID - 10.1182/blood-2001-11-0097 [doi]
PST - ppublish
SO  - Blood. 2002 Dec 15;100(13):4512-20. doi: 10.1182/blood-2001-11-0097. Epub 2002 
      Aug 15.