PMID- 12393733
OWN - NLM
STAT- MEDLINE
DCOM- 20030318
LR  - 20240109
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 101
IP  - 1
DP  - 2003 Jan 1
TI  - Differential distribution and internal translation efficiency of hepatitis C 
      virus quasispecies present in dendritic and liver cells.
PG  - 52-7
AB  - Hepatitis C virus (HCV) is predominantly a hepatotropic virus. Nonetheless, there 
      is mounting evidence that hematopoietic cells may support HCV replication. The 
      HCV 5' untranslated region (5'UTR), responsible for initiation of viral 
      translation, via an internal ribosome entry site (IRES), has been previously 
      described to contain specific nucleotide substitutions when cultured in infected 
      lymphoid cells. Our purpose was to establish whether the 5'UTR polymorphism of 
      quasispecies from 3 cell compartments (liver, peripheral blood mononuclear cells 
      [PBMG], and monocyte-derived dendritic cells [DCs]) of a patient chronically 
      infected with HCV1b affects the corresponding translational efficiencies and thus 
      the capacity for replication. The 5'UTR polymorphism was characterized by 
      identification of changes at 3 crucial sites as compared with the reference 
      nucleotide (nt) sequence: a G insertion between positions 19 and 20, a C>A 
      substitution at position 204 and a G>A substitution at position 243. The 
      quasispecies detected in DCs was unique and differed from those present in the 
      liver, suggesting a particular tropism of HCV quasispecies for DCs. Moreover, its 
      translational activity was significantly impaired when compared with those from 
      liver and PBMCs in different cell lines. This impairment was thoroughly confirmed 
      in primary cultures of both human hepatocytes and monocyte-derived DCs. Taken 
      together, our data lend support both to a specific location and impaired 
      replication of HCV quasispecies in DCs, which could be related to viral 
      persistence and perturbation of DC function in chronically infected patients.
FAU - Laporte, Julien
AU  - Laporte J
AD  - Laboratoire de virologie, C.E.R.VI., UPRES EA 2387, Hopital Pitie-Salpetriere, 
      Paris, France.
FAU - Bain, Christine
AU  - Bain C
FAU - Maurel, Patrick
AU  - Maurel P
FAU - Inchauspe, Genevieve
AU  - Inchauspe G
FAU - Agut, Henri
AU  - Agut H
FAU - Cahour, Annie
AU  - Cahour A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20020628
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (5' Untranslated Regions)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - 5' Untranslated Regions/genetics
MH  - Blood Cells/virology
MH  - Dendritic Cells/*virology
MH  - Female
MH  - *Genetic Variation
MH  - Hepacivirus/*genetics
MH  - Hepatitis C/pathology/virology
MH  - Hepatocytes/*virology
MH  - Humans
MH  - Middle Aged
MH  - Point Mutation
MH  - *Protein Biosynthesis
MH  - RNA, Viral/genetics
MH  - Tissue Distribution
MH  - Tumor Cells, Cultured
EDAT- 2002/10/24 04:00
MHDA- 2003/03/19 04:00
CRDT- 2002/10/24 04:00
PHST- 2002/10/24 04:00 [pubmed]
PHST- 2003/03/19 04:00 [medline]
PHST- 2002/10/24 04:00 [entrez]
AID - S0006-4971(20)53561-1 [pii]
AID - 10.1182/blood-2002-03-0818 [doi]
PST - ppublish
SO  - Blood. 2003 Jan 1;101(1):52-7. doi: 10.1182/blood-2002-03-0818. Epub 2002 Jun 28.