PMID- 12394184
OWN - NLM
STAT- MEDLINE
DCOM- 20030501
LR  - 20191025
IS  - 0960-8931 (Print)
IS  - 0960-8931 (Linking)
VI  - 12
IP  - 5
DP  - 2002 Oct
TI  - Tyr-TGFalpha transgenic mice develop ocular melanocytic lesions.
PG  - 435-9
AB  - Transforming growth factor-alpha (TGFalpha) has been implicated in melanocyte 
      transformation, as it is expressed in melanocytic lesions and in melanoma cells. 
      We investigated its role in melanoma development using a transgenic mouse model. 
      The mice were generated by microinjection of a transgene with 270 bp of the mouse 
      tyrosinase promoter and the cDNA for human TGFalpha. No significant skin 
      abnormalities were found, but individuals from three transgenic lines developed 
      ocular melanocytoses (seven out of 10 transgenics), usually after a long latency 
      period. In particular, the melanocyte component of the choroid was thicker than 
      in non-transgenic controls, consistent with hyperplasia. The retinal pigment 
      epithelium was unaffected. Melanocytic lesions were also present in the posterior 
      eye, and abnormal distributions of melanocytes were found in neural tissue of the 
      brain, skeletal muscle of the head and the Harderian glands, indicating migration 
      from the choroid. It was concluded that mice engineered to express the normal 
      growth factor TGFalpha from a tyrosinase promoter spontaneously developed 
      melanocytic lesions in the eye but not the skin.
FAU - Sutton, R
AU  - Sutton R
AD  - School of Science, University of Western Sydney, Penrith South, NSW, Australia.
FAU - Gordon-Thomson, C
AU  - Gordon-Thomson C
FAU - Cree, I A
AU  - Cree IA
FAU - Mason, R S
AU  - Mason RS
FAU - Moore, G P
AU  - Moore GP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Melanoma Res
JT  - Melanoma research
JID - 9109623
RN  - 0 (Transforming Growth Factor alpha)
RN  - EC 1.14.18.1 (Monophenol Monooxygenase)
SB  - IM
MH  - Animals
MH  - Cell Transformation, Neoplastic
MH  - Eye/metabolism
MH  - Eye Neoplasms/*genetics
MH  - Melanocytes/*metabolism
MH  - Melanoma/*genetics/metabolism
MH  - Mice
MH  - Mice, Transgenic
MH  - Models, Genetic
MH  - Monophenol Monooxygenase/genetics
MH  - Promoter Regions, Genetic
MH  - Tissue Distribution
MH  - Transforming Growth Factor alpha/*genetics
MH  - Transgenes
EDAT- 2002/10/24 04:00
MHDA- 2003/05/02 05:00
CRDT- 2002/10/24 04:00
PHST- 2002/10/24 04:00 [pubmed]
PHST- 2003/05/02 05:00 [medline]
PHST- 2002/10/24 04:00 [entrez]
AID - 10.1097/00008390-200209000-00004 [doi]
PST - ppublish
SO  - Melanoma Res. 2002 Oct;12(5):435-9. doi: 10.1097/00008390-200209000-00004.