PMID- 12396721
OWN - NLM
STAT- MEDLINE
DCOM- 20030324
LR  - 20061115
IS  - 1079-9907 (Print)
IS  - 1079-9907 (Linking)
VI  - 22
IP  - 9
DP  - 2002 Sep
TI  - Cloning, expression analyses, and chromosomal location of porcine interleukin-18 
      receptor alpha chain (IL-18Ralpha).
PG  - 995-1002
AB  - We cloned and sequenced a cDNA that contains the coding sequence of the porcine 
      interleukin-18 receptor alpha chain (PoIL-18Ralpha). Based on the conserved 
      nucleotide sequences between human (HuIL-18Ralpha) and murine IL-18Ralpha 
      (MuIL-18Ralpha), we performed reverse transcription-polymerase chain reaction 
      (RT-PCR) with total RNA prepared from porcine peripheral blood lymphocytes (PBLs) 
      stimulated with PoIL-12 to clone the cDNA of PoIL-18Ralpha. The open reading 
      frame (ORF) of the PoIL-18Ralpha cDNA is 1620 base pairs (bp) in length and 
      encodes 539 amino acids. The predicted amino acid sequence showed 68.2% and 50.2% 
      identity to the human and murine amino acid sequences, respectively. Stimulation 
      with concanavalin A (ConA) and IL-12, but not with IL-4, was shown to upregulate 
      the expression of IL-18Ralpha mRNA in pig PBLs by RT-PCR analysis. Flow 
      cytometric analysis also demonstrated that IL-18Ralpha was constitutively 
      expressed on PoPBLs, and this expression was augmented by ConA stimulation. 
      Furthermore, the PoIL-18Ralpha gene was mapped by fluorescence in situ 
      hybridization (FISH) to porcine chromosome 3 (3q13-q14), near the location at 
      which the IL-1beta gene had already been mapped. The present results will be 
      helpful for understanding PoIL-18 and interferon gamma (IFN-gamma)-mediated T 
      helper 1 (Th1) cell development.
FAU - Muneta, Yoshihiro
AU  - Muneta Y
AD  - Department of Immunology, National Institute of Animal Health, Tsukuba 305-0856, 
      Japan. ymuneta@affrc.go.jp
FAU - Uenishi, Hirohide
AU  - Uenishi H
FAU - Yamamoto, Ryuji
AU  - Yamamoto R
FAU - Yoshihara, Kazuhiro
AU  - Yoshihara K
FAU - Yasue, Hiroshi
AU  - Yasue H
FAU - Awata, Takashi
AU  - Awata T
FAU - Mori, Yasuyuki
AU  - Mori Y
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Interferon Cytokine Res
JT  - Journal of interferon & cytokine research : the official journal of the 
      International Society for Interferon and Cytokine Research
JID - 9507088
RN  - 0 (DNA, Complementary)
RN  - 0 (IL18R1 protein, human)
RN  - 0 (Il18r1 protein, mouse)
RN  - 0 (Interleukin-18 Receptor alpha Subunit)
RN  - 0 (Protein Subunits)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Interleukin)
RN  - 0 (Receptors, Interleukin-18)
RN  - 11028-71-0 (Concanavalin A)
RN  - 187348-17-0 (Interleukin-12)
RN  - 207137-56-2 (Interleukin-4)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Chromosome Mapping/*veterinary
MH  - Cloning, Molecular
MH  - Concanavalin A/pharmacology
MH  - DNA, Complementary/genetics/isolation & purification
MH  - Gene Expression Regulation/*drug effects
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interleukin-12/pharmacology
MH  - Interleukin-18 Receptor alpha Subunit
MH  - Interleukin-4/pharmacology
MH  - Lymphocytes/drug effects/metabolism
MH  - Mice
MH  - Molecular Sequence Data
MH  - Open Reading Frames
MH  - Protein Subunits/genetics
MH  - RNA, Messenger/biosynthesis
MH  - Radiation Hybrid Mapping
MH  - Receptors, Interleukin/biosynthesis/*genetics
MH  - Receptors, Interleukin-18
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Species Specificity
MH  - Swine/*genetics
EDAT- 2002/10/25 04:00
MHDA- 2003/03/26 04:00
CRDT- 2002/10/25 04:00
PHST- 2002/10/25 04:00 [pubmed]
PHST- 2003/03/26 04:00 [medline]
PHST- 2002/10/25 04:00 [entrez]
AID - 10.1089/10799900260286704 [doi]
PST - ppublish
SO  - J Interferon Cytokine Res. 2002 Sep;22(9):995-1002. doi: 
      10.1089/10799900260286704.