PMID- 12397373 OWN - NLM STAT- MEDLINE DCOM- 20030711 LR - 20161124 IS - 0302-766X (Print) IS - 0302-766X (Linking) VI - 310 IP - 2 DP - 2002 Nov TI - Expression patterns of neurotrophic factor mRNAs in developing human teeth. PG - 177-87 AB - Neurotrophic factors regulate survival, differentiation, growth and plasticity in the nervous system. In addition, based on their specific and shifting temporospatial expression patterns, neurotrophic factors have been implicated in morphogenetic events during tooth development in rodents. To determine whether these findings in rodents could be related to humans, we have now studied nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), neurotrophin-4 (NT-4), glial cell-line derived neurotrophic factor (GDNF), and neurturin (NTN) mRNA expression patterns in developing human teeth during gestational weeks 6.5-11. Using in situ hybridization histochemistry, we found distinct and specific patterns of neurotrophin and GDNF mRNA expression in the developing human teeth. NGF mRNA labeling was weak and confined predominantly to the dental papilla. BDNF mRNA labeling was stronger than NGF mRNA and was seen in the mesenchyme located lateral to the dental organ, as well as in epithelial structures (inner dental epithelium and enamel knot). NT-3 mRNA was observed in the dental papilla and in the area of the cervical loop. NT-4 mRNA was expressed in both oral and dental epithelia in all stages studied. GDNF mRNA was found in the dental follicle and at different sites in the inner dental epithelium. Weak NTN mRNA labeling was also found in the developing teeth. Based on these findings, we suggest that neurotrophins, GDNF and NTN might be involved in morphogenetic events during early stages of tooth development in humans. Protein gene product (PGP) 9.5-immunoreactive nerve fibers were observed in the dental follicle by 11 weeks coinciding with the labeling for neurotrophic factor mRNAs in this structure. This suggests that these neurotrophic factors might be involved in the innervation of dental structures. The rich expression of neurotrophic factors in developing dental tissues suggests that developing, or possibly adult, dental tissue might be used as an allograft source of trophic support for diseases of the nervous system. FAU - Nosrat, Irina AU - Nosrat I AD - Laboratory of Oral Neurobiology, University of Michigan Dental School, Ann Arbor, MI 48109-1078, USA. inosrat@umich.edu FAU - Seiger, Ake AU - Seiger A FAU - Olson, Lars AU - Olson L FAU - Nosrat, Christopher A AU - Nosrat CA LA - eng PT - Journal Article DEP - 20020830 PL - Germany TA - Cell Tissue Res JT - Cell and tissue research JID - 0417625 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (GDNF protein, human) RN - 0 (Glial Cell Line-Derived Neurotrophic Factor) RN - 0 (NRTN protein, human) RN - 0 (Nerve Growth Factors) RN - 0 (Neurotrophin 3) RN - 0 (Neurturin) RN - 0 (RNA, Messenger) RN - 9061-61-4 (Nerve Growth Factor) RN - EC 3.1.2.- (Thiolester Hydrolases) RN - EC 3.4.19.12 (Ubiquitin Thiolesterase) RN - P658DCA9XD (neurotrophin 4) SB - IM MH - Brain-Derived Neurotrophic Factor/genetics MH - Gestational Age MH - Glial Cell Line-Derived Neurotrophic Factor MH - Humans MH - Immunohistochemistry MH - Nerve Growth Factor/genetics MH - Nerve Growth Factors/genetics MH - Neurotrophin 3/*genetics MH - Neurturin MH - Odontogenesis/*genetics MH - RNA, Messenger/analysis MH - Thiolester Hydrolases/metabolism MH - Tooth/*embryology/metabolism MH - Ubiquitin Thiolesterase EDAT- 2002/10/25 04:00 MHDA- 2003/07/12 05:00 CRDT- 2002/10/25 04:00 PHST- 2001/12/14 00:00 [received] PHST- 2002/07/12 00:00 [accepted] PHST- 2002/10/25 04:00 [pubmed] PHST- 2003/07/12 05:00 [medline] PHST- 2002/10/25 04:00 [entrez] AID - 10.1007/s00441-002-0618-8 [doi] PST - ppublish SO - Cell Tissue Res. 2002 Nov;310(2):177-87. doi: 10.1007/s00441-002-0618-8. Epub 2002 Aug 30.