PMID- 12398214
OWN - NLM
STAT- MEDLINE
DCOM- 20030224
LR  - 20191210
IS  - 1438-4221 (Print)
IS  - 1438-4221 (Linking)
VI  - 292
IP  - 3-4
DP  - 2002 Sep
TI  - Evaluation of detection of Legionella spp. in water samples by fluorescence in 
      situ hybridization, PCR amplification and bacterial culture.
PG  - 241-5
AB  - One hundred water samples (32 from clinical units and 68 from private households) 
      were examined for Legionella by culture, fluorescence in situ hybridization 
      (FISH) and polymerase chain reaction (PCR). Twenty-four samples were positive by 
      culture (22 L. pneumophila; 2 non-pneumophila species), 36 by FISH (32 L. 
      pneumophila; 4 non-pneumophila species) and 75 by PCR (41 positive for L. 
      pneumophila; 26 positive for L. pneumophila and a non-pneumophila species; 8 
      positive for non-pneumophila species). PCR and FISH results were compared to 
      bacterial culture as the "gold standard" method by calculating sensitivities and 
      specificities, respectively: PCR assays, 96% and 47%; FISH assays, 67% and 72%, 
      respectively. In comparison with FISH the lower specificity of PCR is probably 
      caused by dead Legionella bacteria and/or free Legionella DNA in potable water, 
      and the higher sensitivity of PCR may be explained by the detection limit of 
      fluorescence microscopy. In conclusion, the relatively high specificity, 
      sensitivity and quickness of the FISH assay offer significant advantages over 
      conventional PCR and culture-based techniques.
FAU - Buchbinder, Susanne
AU  - Buchbinder S
AD  - Max-von-Pettenkofer-Institut fur Hygiene und Medizinische Mikrobiologie, Ludwig 
      Maximilians Universitat Munchen, Germany.
FAU - Trebesius, Karlheinz
AU  - Trebesius K
FAU - Heesemann, Jurgen
AU  - Heesemann J
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PL  - Germany
TA  - Int J Med Microbiol
JT  - International journal of medical microbiology : IJMM
JID - 100898849
SB  - IM
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Legionella/genetics/*isolation & purification
MH  - Polymerase Chain Reaction
MH  - Sensitivity and Specificity
MH  - *Water Microbiology
MH  - *Water Supply/standards
EDAT- 2002/10/26 04:00
MHDA- 2003/02/25 04:00
CRDT- 2002/10/26 04:00
PHST- 2002/10/26 04:00 [pubmed]
PHST- 2003/02/25 04:00 [medline]
PHST- 2002/10/26 04:00 [entrez]
AID - S1438-4221(04)70102-2 [pii]
AID - 10.1078/1438-4221-00213 [doi]
PST - ppublish
SO  - Int J Med Microbiol. 2002 Sep;292(3-4):241-5. doi: 10.1078/1438-4221-00213.