PMID- 12398809
OWN - NLM
STAT- MEDLINE
DCOM- 20030404
LR  - 20190922
IS  - 0143-4004 (Print)
IS  - 0143-4004 (Linking)
VI  - 23
IP  - 10
DP  - 2002 Nov
TI  - Transforming growth factor-beta1 regulates hepatocyte growth factor-induced 
      trophoblast motility and invasion.
PG  - 699-705
AB  - During placental development extravillous trophoblasts invade the uterine wall in 
      a tightly regulated manner dependent on both pro- and anti-invasive molecules. We 
      have shown using the extravillous trophoblast cell line, SGHPL-4, that both 
      cellular invasion and motility are stimulated by hepatocyte growth factor (HGF). 
      It has previously been demonstrated that transforming growth factor=beta1 
      (TGF-beta1), produced by the decidua, inhibits extravillous trophoblast 
      proliferation and invasion. It was the aim of this study to determine whether 
      TGF-beta1 could modulate HGF-induced motility and invasion and, if so, examine 
      the mechanism involved. TGF-beta1 significantly inhibited the growth of SGHPL-4 
      cells stimulated with 10 per cent serum. HGF-stimulated trophoblast cell invasion 
      and motility were significantly inhibited by TGF-beta1. Neither HGF nor TGF-beta1 
      had an effect on SGHPL-4 cell growth under the conditions used for the invasion 
      and motility experiments (0.5 per cent serum). Previous studies suggest that both 
      HGF-stimulated trophoblast invasion and motility may be regulated by the 
      production of nitric oxide. TGF-beta1 was found to significantly decrease 
      HGF-induced iNOS expression therefore suggesting a novel mechanism by which 
      TGF-beta1 could regulate motility and invasion.
CI  - Copyright 2002 Elsevier Science Ltd.
FAU - Tse, W K
AU  - Tse WK
AD  - Department of Biochemistry and Immunology, St George's Hospital Medical School, 
      Cranmer Terrace, London, SW17 0RE, UK.
FAU - Whitley, G St J
AU  - Whitley GS
FAU - Cartwright, J E
AU  - Cartwright JE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Placenta
JT  - Placenta
JID - 8006349
RN  - 0 (Culture Media)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (TGFB1 protein, human)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 1.13.12.- (Luciferases)
RN  - EC 1.14.13.39 (NOS2 protein, human)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
SB  - IM
MH  - Blotting, Western
MH  - Cell Division
MH  - Cell Line
MH  - Cell Movement
MH  - Culture Media
MH  - Female
MH  - Fetal Blood
MH  - Gene Expression
MH  - Hepatocyte Growth Factor/*pharmacology
MH  - Humans
MH  - Luciferases/genetics
MH  - Nitric Oxide Synthase/analysis/genetics
MH  - Nitric Oxide Synthase Type II
MH  - Pregnancy
MH  - Promoter Regions, Genetic
MH  - Recombinant Fusion Proteins
MH  - Transfection
MH  - Transforming Growth Factor beta/*pharmacology
MH  - Transforming Growth Factor beta1
MH  - Trophoblasts/cytology/*physiology
EDAT- 2002/10/26 04:00
MHDA- 2003/04/05 05:00
CRDT- 2002/10/26 04:00
PHST- 2002/10/26 04:00 [pubmed]
PHST- 2003/04/05 05:00 [medline]
PHST- 2002/10/26 04:00 [entrez]
AID - S0143400402908660 [pii]
AID - 10.1016/s0143-4004(02)90866-0 [doi]
PST - ppublish
SO  - Placenta. 2002 Nov;23(10):699-705. doi: 10.1016/s0143-4004(02)90866-0.