PMID- 12402192
OWN - NLM
STAT- MEDLINE
DCOM- 20021209
LR  - 20041117
IS  - 0022-1899 (Print)
IS  - 0022-1899 (Linking)
VI  - 186
IP  - 9
DP  - 2002 Nov 1
TI  - Molecular analysis of T cell clonotypes in muscle-infiltrating lymphocytes from 
      patients with human T lymphotropic virus type 1 polymyositis.
PG  - 1231-41
AB  - Epidemiological studies have shown that a correlation may exist between human T 
      cell lymphotropic virus type 1 (HTLV-1) infection and a form of polymyositis 
      (PM). To characterize muscle-infiltrating lymphocytes (MILs) from patients with 
      HTLV-1 PM, we examined the T cell receptor (TCR) beta-chain variable region 
      repertoire and clonotype of MILs and peripheral blood mononuclear cells (PBMC) 
      from 3 patients, using TCR complementarity-determining region 3 (CDR3) length 
      spectratyping and DNA sequencing. Immunohistochemical studies showed that MILs 
      from patients with HTLV-1 PM contain both CD4(+) and CD8(+) T cells. Although 
      some clonotypes observed in PBMC were also found in MILs in all patients 
      examined, MILs consisted predominantly of locally expanded clones. One clonotype 
      in MILs was derived from human leukocyte antigen (HLA)-A*02/Tax11-19 
      tetramer-positive cells, the CDR3 motif of which contains amino acid residues for 
      HLA-A*02/Tax peptide-TCR interaction. We conclude that certain T cell clones 
      proliferate in the muscle lesions of HTLV-1 PM and may contribute to the 
      pathogenesis of the disease.
FAU - Saito, Mineki
AU  - Saito M
AD  - Third Department of Internal Medicine, Faculty of Medicine, Kagoshima University, 
      Sakuragaoka, Kagoshima, Japan. mineki@m3.kufm.kagoshima-u.ac.jp
FAU - Higuchi, Itsuro
AU  - Higuchi I
FAU - Saito, Akiko
AU  - Saito A
FAU - Izumo, Shuji
AU  - Izumo S
FAU - Usuku, Koichiro
AU  - Usuku K
FAU - Bangham, Charles R M
AU  - Bangham CR
FAU - Osame, Mitsuhiro
AU  - Osame M
LA  - eng
PT  - Journal Article
DEP - 20021007
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
RN  - 0 (Receptor-CD3 Complex, Antigen, T-Cell)
RN  - 0 (Receptors, Antigen, T-Cell, alpha-beta)
SB  - IM
MH  - Aged
MH  - Amino Acid Sequence
MH  - CD4-Positive T-Lymphocytes/immunology/pathology
MH  - CD8-Positive T-Lymphocytes/immunology/pathology
MH  - Female
MH  - HTLV-I Infections/*immunology
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Muscle, Skeletal/pathology/virology
MH  - Polymyositis/*immunology/*virology
MH  - Receptor-CD3 Complex, Antigen, T-Cell/chemistry/genetics
MH  - Receptors, Antigen, T-Cell, alpha-beta/chemistry/genetics
MH  - T-Lymphocytes/*immunology
EDAT- 2002/10/29 04:00
MHDA- 2002/12/10 04:00
CRDT- 2002/10/29 04:00
PHST- 2002/04/17 00:00 [received]
PHST- 2002/06/20 00:00 [revised]
PHST- 2002/10/29 04:00 [pubmed]
PHST- 2002/12/10 04:00 [medline]
PHST- 2002/10/29 04:00 [entrez]
AID - JID020434 [pii]
AID - 10.1086/344315 [doi]
PST - ppublish
SO  - J Infect Dis. 2002 Nov 1;186(9):1231-41. doi: 10.1086/344315. Epub 2002 Oct 7.