PMID- 12402381
OWN - NLM
STAT- MEDLINE
DCOM- 20021210
LR  - 20171116
IS  - 0513-5796 (Print)
IS  - 0513-5796 (Linking)
VI  - 43
IP  - 5
DP  - 2002 Oct
TI  - Expression patterns of cytokines and chemokines genes in human hepatoma cells.
PG  - 657-64
AB  - Various cytokines and chemokines play a role in carcinogenesis. However, no study 
      has previously been undertaken to investigate comprehensively the expressions of 
      cytokines and chemokines in hepatoma cells. In this study, we determined which 
      cytokines and chemokines are expressed in hepatoma cells. Recently, it was 
      reported that the expressions of several chemokines could be increased by Fas 
      stimulus in many normal and cancer cells. Therefore, we also investigated whether 
      chemokines expression is regulated by Fas ligation. To address this issue, we 
      performed RNase protection assays upon 13 cytokines and 8 chemokines genes in 10 
      human hepatoma cell lines, comprising 8 hepatitis B virus (HBV)-associated 
      hepatoma cell lines. Transforming growth factor-beta2 (TGF-beta2) was found to be 
      expressed in 8 HBV-associated hepatoma cell lines, and to be potently expressed 
      in 5 cell lines; however, the mRNA expressions of interleukin-10 (IL-10), IL-12, 
      interferon-gamma(IFN-gamma) and tumor necrosis factor-alpha(TNF-alpha) were not 
      detected in any cell lines examined. Among the chemokines investigated in this 
      study, IL-8 was expressed by 8 HBV- associated hepatoma cell lines, and monocyte 
      chemoattractant protein-1 (MCP-1) by 7 HBV-associated hepatoma cell lines. 
      However, the mRNA expressions of macrophage inflammatory 
      protein-1alpha(MIP-1alpha), MIP-1beta, interferon-inducible protein-10 (IP-10), 
      RANTES, lymphotactin and I-309 were either very weak or undetectable. Fas 
      ligation did not increase chemokines expression in hepatoma cells. Conclusively, 
      TGF-beta2, IL-8 and MCP-1 were overexpressed in HBV-associated hepatoma cells, 
      and the expressions of chemokines were not increased by Fas ligation in human 
      hepatoma cells.
FAU - Shin, Eui-Cheol
AU  - Shin EC
AD  - Department of Microbiology, Yonsei University College of Medicine, Seoul, Korea.
FAU - Choi, Youn-Hee
AU  - Choi YH
FAU - Kim, Ji Su
AU  - Kim JS
FAU - Kim, Se Jong
AU  - Kim SJ
FAU - Park, Jeon Han
AU  - Park JH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Korea (South)
TA  - Yonsei Med J
JT  - Yonsei medical journal
JID - 0414003
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (RNA, Messenger)
RN  - 0 (fas Receptor)
SB  - IM
MH  - Carcinoma, Hepatocellular/*metabolism
MH  - Chemokines/*genetics
MH  - Cytokines/*genetics
MH  - Gene Expression Profiling
MH  - Humans
MH  - Liver Neoplasms/*metabolism
MH  - RNA, Messenger/analysis
MH  - Tumor Cells, Cultured
MH  - fas Receptor/physiology
EDAT- 2002/10/29 04:00
MHDA- 2002/12/11 04:00
CRDT- 2002/10/29 04:00
PHST- 2002/10/29 04:00 [pubmed]
PHST- 2002/12/11 04:00 [medline]
PHST- 2002/10/29 04:00 [entrez]
AID - 200210657 [pii]
AID - 10.3349/ymj.2002.43.5.657 [doi]
PST - ppublish
SO  - Yonsei Med J. 2002 Oct;43(5):657-64. doi: 10.3349/ymj.2002.43.5.657.