PMID- 12402581
OWN - NLM
STAT- MEDLINE
DCOM- 20021127
LR  - 20151119
IS  - 1197-8554 (Print)
IS  - 1197-8554 (Linking)
VI  - 18
DP  - 2002
TI  - Glucose suppresses peritoneal inflammatory reactions and mesothelial hyperplasia 
      caused by intraperitoneal saline infusion.
PG  - 21-5
AB  - In the past, we had observed that infusion of normal saline into the peritoneal 
      cavity stimulates an inflammatory response. In the present study, we examined 
      what effect the addition of glucose to normal saline would have on the peritoneal 
      inflammatory response and change in peritoneal morphology. After catheter 
      implantation, rats were infused intraperitoneally (i.p.) for 3 days with Dianeal 
      1.36% (Baxter Healthcare Corporation, Deerfield, IL, U.S.A.). Dialysate samples 
      were collected on day 3 after a 4-hour dwell. Next, rats were exposed to either 
      NaCl (n = 7) or NaCl with glucose 250 mmol/L (Glu, n = 7) twice daily for 4 
      weeks. After 2 weeks and 4 weeks of the study, dialysate samples were collected 
      after a 4-hour dwell to analyze the activity of inflammatory reaction. At the end 
      of the experiment, imprints of peritoneal mesothelium were taken. Control animals 
      (C, n = 6) did not undergo catheter implantation or the dialysis procedure. The 
      inflammatory reaction--cell count, cell differentiation, nitric oxide production, 
      protein loss, and monocyte chemoattractant protein-1 (MCP-1) concentration in 
      dialysate expressed as a percentage of the initial value--did not change during 
      the study in rats exposed to NaCl. On the other hand, in Glu-treated animals, the 
      protein concentration was decreased after 4 weeks of the study (74% +/- 23%, p < 
      0.05), as was MCP-1 (24% +/- 12%, p < 0.05). The nitrites concentration was 
      decreased after 2 weeks (72% +/- 19%; p < 0.05). Intraperitoneal adhesions were 
      found in 6 rats of the NaCl group (86%) and in only 4 rats (57%) of Glu group. In 
      the NaCl rats, a higher density of mesothelial cells was observed (2792 +/- 510 
      cells/mm2) as compared with Glu rats (2028 +/- 561 cells/mm2; p < 0.05) and with 
      control rats (1629 +/- 422 cells/mm2, p < 0.05). The NaCl group also showed a 
      higher nucleus: cytoplasm surface ratio (0.25 +/- 0.03) as compared with the Glu 
      group (0.18 +/- 0.02, p < 0.01) and with the control group (0.14 +/- 0.01, p < 
      0.01). Addition of glucose to normal saline suppresses the peritoneal 
      inflammatory response and mesothelial hyperplasia occurring with intraperitoneal 
      infusion of NaCl solution alone.
FAU - Styszynski, Arkadiusz
AU  - Styszynski A
AD  - Department of Pathophysiology, University Medical School of Poznan, Poland.
FAU - Podkowka, Renata
AU  - Podkowka R
FAU - Wieczorowska-Tobis, Katarzyna
AU  - Wieczorowska-Tobis K
FAU - Kwiatkowska, Beata
AU  - Kwiatkowska B
FAU - Ksiazek, Krzysztof
AU  - Ksiazek K
FAU - Breborowicz, Andrzej
AU  - Breborowicz A
FAU - Oreopoulos, Dimitrios G
AU  - Oreopoulos DG
LA  - eng
PT  - Journal Article
PL  - Canada
TA  - Adv Perit Dial
JT  - Advances in peritoneal dialysis. Conference on Peritoneal Dialysis
JID - 9104803
RN  - 0 (Dialysis Solutions)
RN  - 0 (Isotonic Solutions)
RN  - 0 (Nitrites)
RN  - 0 (Proteins)
RN  - 451W47IQ8X (Sodium Chloride)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Cell Count
MH  - Dialysis Solutions/*toxicity
MH  - Epithelium/drug effects/pathology
MH  - Glucose/*pharmacology
MH  - Hyperplasia
MH  - Inflammation/chemically induced
MH  - Infusions, Parenteral
MH  - Isotonic Solutions
MH  - Macrophages/pathology
MH  - Male
MH  - Neutrophils/pathology
MH  - Nitrites/metabolism
MH  - Peritoneum/*drug effects/metabolism/pathology
MH  - Proteins/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sodium Chloride/*toxicity
EDAT- 2002/10/31 04:00
MHDA- 2002/11/28 04:00
CRDT- 2002/10/31 04:00
PHST- 2002/10/31 04:00 [pubmed]
PHST- 2002/11/28 04:00 [medline]
PHST- 2002/10/31 04:00 [entrez]
PST - ppublish
SO  - Adv Perit Dial. 2002;18:21-5.