PMID- 12403172
OWN - NLM
STAT- MEDLINE
DCOM- 20030404
LR  - 20190922
IS  - 0016-6707 (Print)
IS  - 0016-6707 (Linking)
VI  - 115
IP  - 2
DP  - 2002 Jun
TI  - Repeated losses of TTAGG telomere repeats in evolution of beetles (Coleoptera).
PG  - 179-87
AB  - We studied the occurrence of (TTAGG)n telomere repeats in 12 species of beetles, 
      representing main lineages of the Coleoptera phylogenetic tree, by Southern 
      hybridization and fluorescence in situ hybridization (FISH). In contrast to other 
      insect orders, beetles were heterogeneous with respect to the occurrence of TTAGG 
      repeats. In addition, the presence or absence of (TTAGG)n motif was irrespective 
      of phylogenetic relationships. In the suborder Polyphaga, six species displayed 
      positive hybridization signals. These were Silpha obscura, Agrilus viridis, 
      Ampedus sanguineus, Stegobium paniceum, Oryzaephilus surinamensis, and 
      Leptinotarsa decemlineata. Whereas negative signals were obtained in three 
      polyphagan species, Geotrupes stercorarius, Thanasimusformicarius, and Sitophilus 
      granarius. In the suborder Adephaga, the TTAGG sequence was present in one 
      species, Graphoderus cinereus, and absent in two species, Orectochilus villosus 
      and Pterostichus oblongopunctatus. We concluded that the telomerase-dependent 
      (TTAGG)n motif had been repeatedly lost in different phylogenetic branches of 
      Coleoptera and probably replaced with another mechanism of telomere elongation. 
      This had to happen at least 5-6 times. The results suggest a predisposition or a 
      backup mechanism of telomere maintenance in the genome of beetles that enabled 
      them to make frequent evolutionary changes in the telomere composition.
FAU - Frydrychova, Radmila
AU  - Frydrychova R
AD  - Institute of Entomology ASCR, Ceske Budejovice, Czech Republic.
FAU - Marec, Frantisek
AU  - Marec F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Genetica
JT  - Genetica
JID - 0370740
SB  - IM
MH  - Animals
MH  - *Biological Evolution
MH  - Blotting, Southern
MH  - Coleoptera/classification/*genetics
MH  - Genes, Insect
MH  - In Situ Hybridization, Fluorescence
MH  - Phylogeny
MH  - *Repetitive Sequences, Nucleic Acid
MH  - Telomere/*genetics
EDAT- 2002/10/31 04:00
MHDA- 2003/04/05 05:00
CRDT- 2002/10/31 04:00
PHST- 2002/10/31 04:00 [pubmed]
PHST- 2003/04/05 05:00 [medline]
PHST- 2002/10/31 04:00 [entrez]
AID - 10.1023/a:1020175912128 [doi]
PST - ppublish
SO  - Genetica. 2002 Jun;115(2):179-87. doi: 10.1023/a:1020175912128.