PMID- 12408615
OWN - NLM
STAT- MEDLINE
DCOM- 20021119
LR  - 20191106
IS  - 0141-1136 (Print)
IS  - 0141-1136 (Linking)
VI  - 54
IP  - 3-5
DP  - 2002 Sep-Dec
TI  - Bacterial killing by Mytilus hemocyte monolayers as a model for investigating the 
      signaling pathways involved in mussel immune defence.
PG  - 547-51
AB  - The signaling pathways involved in mussel immune defence were investigated 
      utilizing a model of killing of Escherichia coli by Mytilus galloprovincialis 
      hemocytes in a co-culture setting. In particular, the role played by different 
      mitogen activated protein kinases (MAPKs) and by the production of eicosanoids 
      were investigated utilising specific cell permeant, pharmacological enzyme 
      inhibitors. Hemocyte pretreatment with the p38 MAPK inhibitor SB203580 
      significantly reduced bacterial killing, whereas PD98059 (an inhibitor of 
      ERK--extracellularly regulated kinase--MAPK activation) had no significant 
      effect. Wortmannin also inhibited bacterial killing, indicating a crucial role 
      for PI3-kinase activation in the immune response. Killing of E. coli was also 
      reduced by inhibitors of both PLA2 and cyclooxygenase activities, indicating that 
      eicosanoid production is involved in mediating the response to bacterial 
      challenge. The results demonstrate that bacterial killing by mussel hemocytes is 
      particularly sensitive to inhibitors of the key steps involved in the 
      transduction of bacterial signals into the host cell. Moreover, these data 
      indicate that the hemocyte bactericidal activity can be suitably utilized not 
      only for identifying the signaling pathways involved in the response to bacterial 
      infection, but also as a potential investigative-toxicology model to test drugs 
      and contaminants for their effect on the overall mussel immune defence.
FAU - Canesi, L
AU  - Canesi L
AD  - Istituto di Scienze Fisiologiche, Universita di Urbino, PS, Italy. 
      canesi@uniurb.it
FAU - Scarpato, A
AU  - Scarpato A
FAU - Betti, M
AU  - Betti M
FAU - Ciacci, C
AU  - Ciacci C
FAU - Pruzzo, C
AU  - Pruzzo C
FAU - Gallo, G
AU  - Gallo G
LA  - eng
PT  - Journal Article
PL  - England
TA  - Mar Environ Res
JT  - Marine environmental research
JID - 9882895
RN  - 0 (Water Pollutants)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Bacterial Infections/*veterinary
MH  - Bivalvia/*immunology
MH  - Cell Culture Techniques
MH  - Escherichia coli
MH  - Hemocytes/*immunology
MH  - Immunity, Cellular/*physiology
MH  - Mitogen-Activated Protein Kinases/*biosynthesis/pharmacology
MH  - Mortality
MH  - Signal Transduction
MH  - Water Pollutants/adverse effects
EDAT- 2002/11/01 04:00
MHDA- 2002/11/26 04:00
CRDT- 2002/11/01 04:00
PHST- 2002/11/01 04:00 [pubmed]
PHST- 2002/11/26 04:00 [medline]
PHST- 2002/11/01 04:00 [entrez]
AID - S0141-1136(02)00144-7 [pii]
AID - 10.1016/s0141-1136(02)00144-7 [doi]
PST - ppublish
SO  - Mar Environ Res. 2002 Sep-Dec;54(3-5):547-51. doi: 10.1016/s0141-1136(02)00144-7.