PMID- 12408642
OWN - NLM
STAT- MEDLINE
DCOM- 20021119
LR  - 20191106
IS  - 0141-1136 (Print)
IS  - 0141-1136 (Linking)
VI  - 54
IP  - 3-5
DP  - 2002 Sep-Dec
TI  - The expression of CYP1A, vitellogenin and zona radiata proteins in Atlantic 
      salmon (Salmo salar) after oral dosing with two commercial PBDE flame retardant 
      mixtures: absence of short-term responses.
PG  - 719-24
AB  - The short-term effects of the commercial PBDE flame retardant mixtures Penta-BDE 
      and cta-BDE on the expression of cytochrome P450 1A (CYP1A), vitellogenin (Vtg) 
      and zona radiata proteins (Zrp) were investigated in juvenile salmon (Salmo 
      salar). For this purpose, groups of fish were dosed twice (oral intake at days I 
      and 4) with 10 and 50 mg/kg body weight of both commercial mixtures. The fishes 
      were sacrificed at day 7 (n = 5 for each group) and 14 (n = 6 for each group), 
      and blood, liver, fillet, and brain were collected. Blanks and positive controls 
      were also part of the experiment. The expressions of Vtg, Zrp, and CYPIA were 
      measured with several techniques (EROD, ELISA, Western, Northern and Slot Blot). 
      The values in the groups of fish treated with Penta-BDE or Octa-BDE did not 
      significantly differ from the reference group for any of the parameters tested. 
      In contrast, the positive control groups treated with estradiol-17beta for Vtg 
      and Zrp expression, and beta-naphthoflavone for CYP1A expression did show a 
      significant response, indicating the potential sensitivity of the fishes for the 
      parameters measured. Since the results of the chemical analyses showed 
      concentrations of a number of PBDE congeners in liver, fillet, and brain that 
      were about three orders of magnitude above those of fish from the North Sea, it 
      is concluded that the short-term toxicity of both commercial PBDE mixtures for 
      these endpoints was low.
FAU - Boon, J P
AU  - Boon JP
AD  - Royal Netherlands Institute for Sea Research (NIOZ), Department of Marine 
      Biogeochemistry and Toxicology, Texel. boon@nioz.nl
FAU - van Zanden, J J
AU  - van Zanden JJ
FAU - Lewis, W E
AU  - Lewis WE
FAU - Zegers, B N
AU  - Zegers BN
FAU - Goksoyr, A
AU  - Goksoyr A
FAU - Arukwe, A
AU  - Arukwe A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Mar Environ Res
JT  - Marine environmental research
JID - 9882895
RN  - 0 (Egg Proteins)
RN  - 0 (Flame Retardants)
RN  - 0 (Halogenated Diphenyl Ethers)
RN  - 0 (Hydrocarbons, Brominated)
RN  - 0 (Phenyl Ethers)
RN  - 0 (Polybrominated Biphenyls)
RN  - 0 (Vitellogenins)
RN  - 0 (Water Pollutants, Chemical)
RN  - 0 (zona radiata protein, fish)
RN  - 4TI98Z838E (Estradiol)
RN  - 7REL09ZX35 (pentabromodiphenyl ether)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Cytochrome P-450 CYP1A1/*biosynthesis
MH  - Egg Proteins/*biosynthesis
MH  - Enzyme-Linked Immunosorbent Assay/veterinary
MH  - Estradiol/biosynthesis
MH  - Flame Retardants/*adverse effects
MH  - *Gene Expression Regulation
MH  - Halogenated Diphenyl Ethers
MH  - Hydrocarbons, Brominated/*adverse effects
MH  - Larva/physiology
MH  - Phenyl Ethers/*adverse effects
MH  - Polybrominated Biphenyls
MH  - Salmo salar/*physiology
MH  - Vitellogenins/*biosynthesis
MH  - Water Pollutants, Chemical/*adverse effects
EDAT- 2002/11/01 04:00
MHDA- 2002/11/26 04:00
CRDT- 2002/11/01 04:00
PHST- 2002/11/01 04:00 [pubmed]
PHST- 2002/11/26 04:00 [medline]
PHST- 2002/11/01 04:00 [entrez]
AID - S0141-1136(02)00127-7 [pii]
AID - 10.1016/s0141-1136(02)00127-7 [doi]
PST - ppublish
SO  - Mar Environ Res. 2002 Sep-Dec;54(3-5):719-24. doi: 10.1016/s0141-1136(02)00127-7.