PMID- 12409386
OWN - NLM
STAT- MEDLINE
DCOM- 20030206
LR  - 20210526
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 40
IP  - 11
DP  - 2002 Nov
TI  - Detection of antibodies directed against human herpesvirus 6 U94/REP in sera of 
      patients affected by multiple sclerosis.
PG  - 4131-7
AB  - The association between human herpesvirus 6 (HHV-6) and multiple sclerosis (MS) 
      is controversial. In fact, it is difficult to establish a causative role of 
      HHV-6, due to the high prevalence of latently infected individuals in the healthy 
      population. Therefore, the presence of virus sequences in tissue biopsy does not 
      support a viral role, and serological assays do not show significant differences 
      between MS patients and control populations. The only viral gene expressed during 
      latency is U94/rep. Therefore, we have developed a serological assay for the 
      detection of antibodies specifically directed against U94/REP protein. Different 
      populations were analyzed by enzyme-linked immunosorbent assay, including healthy 
      controls, MS patients, and subjects with diseases unrelated to HHV-6 infection, 
      including other neurological diseases. The results show statistically significant 
      differences (P > 0.01) between MS patients and control groups, both in antibody 
      prevalence (87 and 43.9%, respectively) and in geometric mean titer (1:515 and 
      1:190, respectively). The detection of antibodies specific for HHV-6 U94/REP 
      shows that the immune system is exposed to this antigen during natural infection. 
      The higher prevalence and higher titers of antibodies to U94/REP suggest that MS 
      patients and control groups might experience different exposures to HHV-6.
FAU - Caselli, Elisabetta
AU  - Caselli E
AD  - Section of Microbiology, Department of Experimental and Diagnostic Medicine. 
      Section of Neurology, Department of Medical Surgical, Communication and 
      Behavioral Sciences, University of Ferrara, Ferrara, Italy.
FAU - Boni, Michela
AU  - Boni M
FAU - Bracci, Arianna
AU  - Bracci A
FAU - Rotola, Antonella
AU  - Rotola A
FAU - Cermelli, Claudio
AU  - Cermelli C
FAU - Castellazzi, Massimiliano
AU  - Castellazzi M
FAU - Di Luca, Dario
AU  - Di Luca D
FAU - Cassai, Enzo
AU  - Cassai E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Antibodies, Viral)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Viral Nonstructural Proteins)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Antibodies, Viral/*blood
MH  - Blotting, Western
MH  - Child
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Herpesvirus 6, Human/*immunology
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Multiple Sclerosis/*virology
MH  - Recombinant Proteins/genetics/immunology
MH  - Roseolovirus Infections/*virology
MH  - Viral Nonstructural Proteins/genetics/*immunology
PMC - PMC139661
EDAT- 2002/11/01 04:00
MHDA- 2003/02/07 04:00
PMCR- 2002/11/01
CRDT- 2002/11/01 04:00
PHST- 2002/11/01 04:00 [pubmed]
PHST- 2003/02/07 04:00 [medline]
PHST- 2002/11/01 04:00 [entrez]
PHST- 2002/11/01 00:00 [pmc-release]
AID - 0505 [pii]
AID - 10.1128/JCM.40.11.4131-4137.2002 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2002 Nov;40(11):4131-7. doi: 10.1128/JCM.40.11.4131-4137.2002.