PMID- 12411062
OWN - NLM
STAT- MEDLINE
DCOM- 20021217
LR  - 20131121
IS  - 0253-2727 (Print)
IS  - 0253-2727 (Linking)
VI  - 23
IP  - 6
DP  - 2002 Jun
TI  - A preliminary study on the early diagnosis of myelodysplastic syndromes.
PG  - 307-10
AB  - OBJECTIVE: To evaluate the four techniques for clonal analysis in the early 
      diagnosis of myelodysplastic syndromes (MDS). METHODS: Four techniques for clonal 
      analysis were performed in bone marrow samples from fifty patients with suspected 
      MDS: (1) Conventional cytogenetics (CC) for clonal chromosomal abnormalities; (2) 
      BrdU-sister chromatid differentiation (BrdU-SCD) for cell cycle analysis; (3) 
      Fluorescence in situ hybridization (FISH) for trisomy 8; (4) PCR-SSCP for N-ras 
      mutation. RESULTS: The diagnosis of forty-five patients was compatible with FAB 
      criteria of MDS, the other five patients didn't fully meet the FAB criteria. They 
      had either only one lineage dyspoiesis or no any obvious dysplastic features and 
      two of them were diagnosed as suspicious refractory anemia (RA), one as anemia 
      with hypercellular bone marrow and two as chronic aplastic anemia. The results of 
      the four techniques performed in them showed that four patients had clonal 
      karyotype abnormalities, two had prolonged cell cycle, three had trisomy 8 of 
      different proportions, and one had N-ras mutation. Thus, they were all diagnosed 
      as RA. CONCLUSION: The untypical MDS patients can be diagnosed early by 
      examination with combining several clonal analysis techniques.
FAU - Qian, Jun
AU  - Qian J
AD  - First Affiliated Hospital, Soochow University, Jiangsu Institute of Hematology, 
      Suzhou 215006, China.
FAU - Xue, Yongquan
AU  - Xue Y
FAU - Yu, Fei
AU  - Yu F
FAU - Wu, Yafang
AU  - Wu Y
FAU - Pan, Jinlan
AU  - Pan J
FAU - Lu, Dingwei
AU  - Lu D
LA  - eng
PT  - Journal Article
PL  - China
TA  - Zhonghua Xue Ye Xue Za Zhi
JT  - Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi
JID - 8212398
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Bromodeoxyuridine
MH  - Child
MH  - Child, Preschool
MH  - Chromatids
MH  - Chromosome Aberrations
MH  - Cytogenetic Analysis/methods
MH  - Female
MH  - Genes, ras
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Male
MH  - Middle Aged
MH  - Mutation
MH  - Myelodysplastic Syndromes/*diagnosis/genetics
EDAT- 2002/11/02 04:00
MHDA- 2002/12/18 04:00
CRDT- 2002/11/02 04:00
PHST- 2002/11/02 04:00 [pubmed]
PHST- 2002/12/18 04:00 [medline]
PHST- 2002/11/02 04:00 [entrez]
PST - ppublish
SO  - Zhonghua Xue Ye Xue Za Zhi. 2002 Jun;23(6):307-10.