PMID- 12411099
OWN - NLM
STAT- MEDLINE
DCOM- 20021122
LR  - 20131121
IS  - 0366-6999 (Print)
IS  - 0366-6999 (Linking)
VI  - 115
IP  - 9
DP  - 2002 Sep
TI  - Glomerular chemokine expression and the effect of steroid and cyclophosphamide 
      pulse therapy in human crescentic glomerulonephritis.
PG  - 1301-7
AB  - OBJECTIVE: To study glomerular expression of C-C chemokines, monocyte 
      chemoattractant protein-1 (MCP-1), macrophage inflammatory protein-1alpha and 
      beta (MIP-1alpha, MIP-1beta) and the effect of steroid and cyclophosphamide (CTX) 
      intermittent intravenous pulse therapy on expression in patients with crescentic 
      glomerulonephritis (CGN) to further investigate the underlying mechanism of the 
      treatment. METHODS: Twelve patients with initial biopsy-proven CGN(2), 6 with 
      lupus nephritis (lupus-CGN, LN-CGN) and 6 with vasculitis, (vasculitis-CGN, 
      V-CGN) were enrolled in this study. They underwent an initial biopsy before 
      steroid and CTX intermittent intravenous pulse therapy and were biopsied again 
      one to three months later. Expression of MCP-1, MIP-1alpha, MIP-1beta, and CD68 
      in glomeruli with cellular and fibrocellular crescents were examined by 
      immunohistochemical analysis in serial sections of renal biopsies. The effect of 
      the pulse therapy on histopathological changes was also observed. RESULTS: 
      Although steroid and CTX intermittent intravenous pulse therapy markedly reduced 
      the degree of glomerular crescent formation both in LN-CGN and V-CGN, the effect 
      of the therapy on glomerular chemokine expression was significantly different 
      between LN-CGN and V-CGN. It was found that steroid and CTX intermittent 
      intravenous pulse therapy reduced the expression of CD68, MCP-1, and MIP-1alpha, 
      but had no effect on MIP-1beta in glomeruli with cellular crescents of patients 
      with LN-CGN. In patients with V-CGN, the therapy also reduced the expression of 
      CD68, but had no effect on MCP-1, MIP-1alpha, and MIP-1beta in glomeruli with 
      cellular crescents. It was noted that the degree of glomerulosclerosis and 
      tubular interstitial fibrosis increased more significantly at the second biopsy 
      in V-CGN as compared to LN-CGN. CONCLUSIONS: The efficacy of steroid and CTX 
      intermittent intravenous pulse therapy in CGN might be affected by reduction of 
      glomerular chemokine expression. The different changes in glomerular expression 
      of MCP-1 and MIP-1alpha in patients with LN-CGN and V-CGN after pulse therapy may 
      correlate to different responses to treatment and prognosis.
FAU - Chen, Shufen
AU  - Chen S
AD  - Nanjing University School of Medicine and Research Institute of Nephrology, 
      Jinling Hospital, China.
FAU - Liu, Zhihong
AU  - Liu Z
FAU - Chen, Huiping
AU  - Chen H
FAU - Zhou, Hong
AU  - Zhou H
FAU - Wang, Jianping
AU  - Wang J
FAU - Li, Leishi
AU  - Li L
LA  - eng
PT  - Journal Article
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
RN  - 0 (Adrenal Cortex Hormones)
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (CD68 antigen, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL3)
RN  - 0 (Chemokine CCL4)
RN  - 0 (Chemokines, CC)
RN  - 0 (Macrophage Inflammatory Proteins)
RN  - 8N3DW7272P (Cyclophosphamide)
SB  - IM
MH  - Adolescent
MH  - Adrenal Cortex Hormones/*administration & dosage
MH  - Adult
MH  - Antigens, CD/analysis
MH  - Antigens, Differentiation, Myelomonocytic/analysis
MH  - Biopsy
MH  - Chemokine CCL2/analysis
MH  - Chemokine CCL3
MH  - Chemokine CCL4
MH  - Chemokines, CC/*analysis
MH  - Child
MH  - Cyclophosphamide/*administration & dosage
MH  - Female
MH  - Glomerulonephritis/*drug therapy/immunology/pathology
MH  - Humans
MH  - Kidney Glomerulus/*chemistry/pathology
MH  - Macrophage Inflammatory Proteins/analysis
MH  - Male
MH  - Middle Aged
EDAT- 2002/11/02 04:00
MHDA- 2002/11/26 04:00
CRDT- 2002/11/02 04:00
PHST- 2002/11/02 04:00 [pubmed]
PHST- 2002/11/26 04:00 [medline]
PHST- 2002/11/02 04:00 [entrez]
PST - ppublish
SO  - Chin Med J (Engl). 2002 Sep;115(9):1301-7.