PMID- 12411539
OWN - NLM
STAT- MEDLINE
DCOM- 20030501
LR  - 20190513
IS  - 0022-3751 (Print)
IS  - 1469-7793 (Electronic)
IS  - 0022-3751 (Linking)
VI  - 544
IP  - 3
DP  - 2002 Nov 1
TI  - Lactate and glucose interactions during rest and exercise in men: effect of 
      exogenous lactate infusion.
PG  - 963-75
AB  - To test the hypothesis that lactate plays a central role in the distribution of 
      carbohydrate (CHO) potential energy for oxidation and glucose production (GP), we 
      performed a lactate clamp (LC) procedure during rest and moderate intensity 
      exercise. Blood [lactate] was clamped at approximately 4 mM by exogenous lactate 
      infusion. Subjects performed 90 min exercise trials at 65 % of the peak rate of 
      oxygen consumption (V(O(2))(,peak); 65 %), 55 % V(O(2))(,peak) (55 %) and 55 % 
      V(O(2))(,peak) with lactate clamped to the blood [lactate] that was measured at 
      65 % V(O(2))(,peak) (55 %-LC). Lactate and glucose rates of appearance (R(a)), 
      disappearance (R(d)) and oxidation (R(ox)) were measured with a combination of 
      [3-(13)C]lactate, H(13)CO(3)(-), and [6,6-(2)H(2)]glucose tracers. During rest 
      and exercise, lactate R(a) and R(d) were increased at 55 %-LC compared to 55 %. 
      Glucose R(a) and R(d) were decreased during 55 %-LC compared to 55 %. Lactate 
      R(ox) was increased by LC during exercise (55 %: 6.52 +/- 0.65 and 55 %-LC: 10.01 
      +/- 0.68 mg kg(-1) min(-1)) which was concurrent with a decrease in glucose 
      oxidation (55 %: 7.64 +/- 0.4 and 55 %-LC: 4.35 +/- 0.31 mg kg(-1) min(-1)). With 
      LC, incorporation of (13)C from tracer lactate into blood glucose (L GNG) 
      increased while both GP and calculated hepatic glycogenolysis (GLY) decreased. 
      Therefore, increased blood [lactate] during moderate intensity exercise increased 
      lactate oxidation, spared blood glucose and decreased glucose production. 
      Further, exogenous lactate infusion did not affect rating of perceived exertion 
      (RPE) during exercise. These results demonstrate that lactate is a useful 
      carbohydrate in times of increased energy demand.
FAU - Miller, Benjamin F
AU  - Miller BF
AD  - Department of Integrative Biology, University of California, Berkeley 94720, USA.
FAU - Fattor, Jill A
AU  - Fattor JA
FAU - Jacobs, Kevin A
AU  - Jacobs KA
FAU - Horning, Michael A
AU  - Horning MA
FAU - Navazio, Franco
AU  - Navazio F
FAU - Lindinger, Michael I
AU  - Lindinger MI
FAU - Brooks, George A
AU  - Brooks GA
LA  - eng
GR  - R01 AR042906/AR/NIAMS NIH HHS/United States
GR  - AR-42906/AR/NIAMS NIH HHS/United States
PT  - Clinical Trial
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Physiol
JT  - The Journal of physiology
JID - 0266262
RN  - 0 (Blood Glucose)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 9005-79-2 (Glycogen)
SB  - IM
MH  - Adult
MH  - Blood Glucose/*metabolism
MH  - Energy Metabolism
MH  - Exercise/*physiology
MH  - Gluconeogenesis
MH  - Glycogen/metabolism
MH  - Heart/physiology
MH  - Humans
MH  - Kinetics
MH  - Lactic Acid/blood/*metabolism/pharmacology
MH  - Liver/metabolism
MH  - Male
MH  - Osmolar Concentration
MH  - Oxidation-Reduction
MH  - Oxygen Consumption
MH  - Reference Values
MH  - Respiration
MH  - Rest/physiology
PMC - PMC2290635
EDAT- 2002/11/02 04:00
MHDA- 2003/05/02 05:00
PMCR- 2003/11/01
CRDT- 2002/11/02 04:00
PHST- 2002/11/02 04:00 [pubmed]
PHST- 2003/05/02 05:00 [medline]
PHST- 2002/11/02 04:00 [entrez]
PHST- 2003/11/01 00:00 [pmc-release]
AID - PHY_027128 [pii]
AID - 10.1113/jphysiol.2002.027128 [doi]
PST - ppublish
SO  - J Physiol. 2002 Nov 1;544(3):963-75. doi: 10.1113/jphysiol.2002.027128.