PMID- 12413735
OWN - NLM
STAT- MEDLINE
DCOM- 20030508
LR  - 20190826
IS  - 0165-2478 (Print)
IS  - 0165-2478 (Linking)
VI  - 84
IP  - 3
DP  - 2002 Dec 3
TI  - Efficacy of oral administration and oral intake of edible vaccines.
PG  - 185-90
AB  - To evaluate whether vaccine administration via intragastric gavage is indicative 
      for the outcome of edible vaccines, mice were orally immunised with ovalbumin 
      (OVA) mixed with or without Vibrio cholerae toxin (CT) in various compositions 
      via various routes: (1) OVA dissolved in saline and intragastrically (IG) 
      administered ('IG'); (2) OVA mixed with food extract and administered IG ('food 
      IG'); (3) food chow absorbed with OVA dissolved in saline and fed to the animals 
      ('food'); and (4) OVA dissolved in saline and administered via drinking bottles 
      ('drinking'). When given to naive mice, 'IG' and 'food IG' but not 'food' or 
      'drinking' induced anti-OVA IgG1 responses in serum, but oral boost immunisations 
      were necessary. Serum IgA was not induced. Oral boosting of subcutaneously (SC) 
      primed mice enhanced the IgG1 and IgA response in serum regardless of the route 
      of immunisation or the vaccine composition. CT did not dramatically enhance the 
      immune response. All immunisation routes except 'drinking' induced 
      antigen-specific IgA antibody secreting cells (ASC) in the lamina propria of 
      naive mice. But antigen-specific antibody responses in faeces were not observed. 
      We concluded that oral (i.e. IG) administration is distinct from oral intake. The 
      composition of the vaccine (food or saline) did not influence oral 
      administration. We thus suggested that the route of administration greatly 
      influenced the outcome of oral immunisation. Although oral administration is a 
      well-accepted route to test the potentials of oral vaccines, our study 
      demonstrated that it is merely indicative for the effectiveness of edible 
      vaccines. Studies on the feasibility of edible vaccines should thus be performed 
      by eating the vaccine.
FAU - Lauterslager, Tosca G M
AU  - Lauterslager TG
AD  - ID-Lelystad B.V., PO Box 65, NL-8200 AB Lelystad, The Netherlands. 
      tgm.lauterslager@vumc.nl
FAU - Hilgers, Luuk A T
AU  - Hilgers LA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - Immunol Lett
JT  - Immunology letters
JID - 7910006
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Enterotoxins)
RN  - 0 (Immunoglobulin A)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Vaccines, Edible)
RN  - 0 (stN protein, Vibrio cholerae)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Antibodies, Bacterial/blood
MH  - Antibody-Producing Cells/immunology
MH  - Drinking
MH  - Enterotoxins/administration & dosage/*immunology/pharmacology
MH  - Female
MH  - Food
MH  - Immunoglobulin A/blood
MH  - Immunoglobulin G/blood
MH  - Mice
MH  - Ovalbumin/administration & dosage/*immunology/pharmacology
MH  - Vaccines, Edible/*administration & dosage/immunology
EDAT- 2002/11/05 04:00
MHDA- 2003/05/09 05:00
CRDT- 2002/11/05 04:00
PHST- 2002/11/05 04:00 [pubmed]
PHST- 2003/05/09 05:00 [medline]
PHST- 2002/11/05 04:00 [entrez]
AID - S0165247802001840 [pii]
AID - 10.1016/s0165-2478(02)00184-0 [doi]
PST - ppublish
SO  - Immunol Lett. 2002 Dec 3;84(3):185-90. doi: 10.1016/s0165-2478(02)00184-0.