PMID- 12414748
OWN - NLM
STAT- MEDLINE
DCOM- 20030415
LR  - 20190508
IS  - 1071-412X (Print)
IS  - 1098-6588 (Electronic)
IS  - 1071-412X (Linking)
VI  - 9
IP  - 6
DP  - 2002 Nov
TI  - Nucleocapsid protein-based enzyme-linked immunosorbent assay for detection and 
      differentiation of antibodies against European and North American porcine 
      reproductive and respiratory syndrome virus.
PG  - 1183-91
AB  - Two types of porcine reproductive and respiratory syndrome virus (PRRSV) have 
      been reported, the European type (EU PRRSV) and the North American type (US 
      PRRSV). We developed a dual enzyme-linked immunosorbent assay (ELISA) for the 
      simultaneous detection and differentiation of serum antibodies directed against 
      either of the two PRRSV types. This tandem PRRS ELISA is based on 
      affinity-purified recombinant nucleocapsid protein expressed in Escherichia coli. 
      Sensitivity and specificity were assessed by using the IDEXX HerdChek PRRS ELISA 
      and the indirect immunofluorescence assay as reference tests. A total of 1571 
      sera originating from the United States, Europe, and two PRRS-free countries, 
      i.e., Switzerland and New Zealand, were used for validation of the tandem PRRS 
      ELISA. The new test performed at least as well as the reference tests in regard 
      to sensitivity (0.94 for the US PRRS ELISA and 0.93 for the EU PRRS ELISA) and 
      specificity (0.96 for the US PRRS ELISA and 0.99 for the EU PRRS ELISA). Positive 
      sera were correctly differentiated in 582 of 591 cases, indicating a high 
      differentiation capability of this dual ELISA. The robustness and repeatability 
      of the test were assessed and found to be appropriate for diagnostic 
      applications. Taken together, the data indicate that the tandem PRRS ELISA 
      described here is the first differentiation ELISA for PRRSV serology based on 
      recombinant antigen. It is convenient with respect to antigen production, and it 
      is reliable, economical, and highly sensitive and specific. Thus, it is 
      considered to be a powerful tool for routine diagnostics, epidemiological 
      surveys, and outbreak investigations.
FAU - Seuberlich, Torsten
AU  - Seuberlich T
AD  - Institute of Virology and Immunoprophylaxis, CH-3147 Mittelhausern, Switzerland.
FAU - Tratschin, Jon-Duri
AU  - Tratschin JD
FAU - Thur, Barbara
AU  - Thur B
FAU - Hofmann, Martin A
AU  - Hofmann MA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Clin Diagn Lab Immunol
JT  - Clinical and diagnostic laboratory immunology
JID - 9421292
RN  - 0 (Antibodies, Viral)
RN  - 0 (Nucleocapsid Proteins)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies, Viral/*blood
MH  - Cloning, Molecular
MH  - Enzyme-Linked Immunosorbent Assay/*methods
MH  - Molecular Sequence Data
MH  - Nucleocapsid Proteins/*immunology/isolation & purification
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Recombinant Proteins/immunology/isolation & purification
MH  - Reproducibility of Results
MH  - Swine
PMC - PMC130101
EDAT- 2002/11/05 04:00
MHDA- 2003/04/16 05:00
PMCR- 2002/11/01
CRDT- 2002/11/05 04:00
PHST- 2002/11/05 04:00 [pubmed]
PHST- 2003/04/16 05:00 [medline]
PHST- 2002/11/05 04:00 [entrez]
PHST- 2002/11/01 00:00 [pmc-release]
AID - 0062 [pii]
AID - 10.1128/cdli.9.6.1183-1191.2002 [doi]
PST - ppublish
SO  - Clin Diagn Lab Immunol. 2002 Nov;9(6):1183-91. doi: 
      10.1128/cdli.9.6.1183-1191.2002.