PMID- 12414753
OWN - NLM
STAT- MEDLINE
DCOM- 20030415
LR  - 20240410
IS  - 1071-412X (Print)
IS  - 1098-6588 (Electronic)
IS  - 1071-412X (Linking)
VI  - 9
IP  - 6
DP  - 2002 Nov
TI  - Detection of DNA of lymphotropic herpesviruses in plasma of human 
      immunodeficiency virus-infected patients: frequency and clinical significance.
PG  - 1222-8
AB  - The frequency and clinical significance of detection of DNA of cytomegalovirus 
      (CMV), Epstein-Barr virus (EBV), human herpesvirus 6 (HHV-6), HHV-7, and HHV-8 in 
      plasma were investigated by PCR. The plasma was obtained from 120 selected human 
      immunodeficiency virus (HIV)-infected patients, of whom 75 had AIDS-related 
      manifestations, 32 had primary HIV infection (PHI), and 13 had asymptomatic 
      infections. Nested PCR analysis revealed that none of the lymphotropic 
      herpesviruses tested were found in patients with PHI, in asymptomatic 
      HIV-positive individuals, or in HIV-negative controls. By contrast, DNA of one or 
      more of the viruses was found in 42 (56%) of 75 patients with AIDS-related 
      manifestations, including CMV disease (CMV-D) or AIDS-related tumors. The 
      presence of CMV DNA in plasma was significantly associated with CMV-D (P < 
      0.001). By contrast, EBV detection was not significantly associated with 
      AIDS-related lymphomas (P = 0.31). Interestingly, the presence of HHV-8 DNA in 
      plasma was significantly associated with Kaposi's sarcoma (KS) disease (P < 
      0.001) and with the clinical status of KS patients (P < 0.001). CMV (primarily), 
      EBV, and HHV-8 were the viruses most commonly reactivated in the context of 
      severe immunosuppression (P < 0.05). In contrast, HHV-6 and HHV-7 infections were 
      infrequent at any stage of disease. In conclusion, plasma PCR was confirmed to be 
      useful in the diagnosis of CMV-D but not in that of tumors or other conditions 
      possibly associated with EBV, HHV-6, and HHV-7. Our findings support the 
      hypothesis of a direct involvement of HHV-8 replication in KS pathogenesis, thus 
      emphasizing the usefulness of sensitive and specific diagnostic tests to monitor 
      HHV-8 infection.
FAU - Broccolo, Francesco
AU  - Broccolo F
AD  - Division of Infectious Diseases, San Raffaele Scientific Institute, Milan, Italy. 
      broccolo.francesco@hsr.it
FAU - Bossolasco, Simona
AU  - Bossolasco S
FAU - Careddu, Anna M
AU  - Careddu AM
FAU - Tambussi, Giuseppe
AU  - Tambussi G
FAU - Lazzarin, Adriano
AU  - Lazzarin A
FAU - Cinque, Paola
AU  - Cinque P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Clin Diagn Lab Immunol
JT  - Clinical and diagnostic laboratory immunology
JID - 9421292
RN  - 0 (DNA, Viral)
SB  - IM
MH  - AIDS-Related Opportunistic Infections/diagnosis
MH  - Adult
MH  - Cytomegalovirus/isolation & purification
MH  - DNA, Viral/*blood
MH  - Female
MH  - HIV Infections/*virology
MH  - Herpesviridae/*isolation & purification
MH  - Herpesviridae Infections/diagnosis
MH  - Herpesvirus 4, Human/isolation & purification
MH  - Herpesvirus 6, Human/isolation & purification
MH  - Herpesvirus 7, Human/isolation & purification
MH  - Herpesvirus 8, Human/isolation & purification
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - Viral Load
PMC - PMC130108
EDAT- 2002/11/05 04:00
MHDA- 2003/04/16 05:00
PMCR- 2002/11/01
CRDT- 2002/11/05 04:00
PHST- 2002/11/05 04:00 [pubmed]
PHST- 2003/04/16 05:00 [medline]
PHST- 2002/11/05 04:00 [entrez]
PHST- 2002/11/01 00:00 [pmc-release]
AID - 0093 [pii]
AID - 10.1128/cdli.9.6.1222-1228.2002 [doi]
PST - ppublish
SO  - Clin Diagn Lab Immunol. 2002 Nov;9(6):1222-8. doi: 
      10.1128/cdli.9.6.1222-1228.2002.