PMID- 12414754
OWN - NLM
STAT- MEDLINE
DCOM- 20030415
LR  - 20190508
IS  - 1071-412X (Print)
IS  - 1098-6588 (Electronic)
IS  - 1071-412X (Linking)
VI  - 9
IP  - 6
DP  - 2002 Nov
TI  - Cytokine profiles in peripheral blood mononuclear cells and lymph node cells from 
      piglets infected in utero with porcine reproductive and respiratory syndrome 
      virus.
PG  - 1229-34
AB  - The aim of the present study was to investigate at 2, 4, and 6 weeks after birth 
      cytokine expression by peripheral blood mononuclear cells and bronchial lymph 
      node cells from piglets infected in utero with porcine reproductive and 
      respiratory syndrome virus (PRRSV). Technically, by flow cytometry we were able 
      to measure gamma interferon (gamma-IFN), tumor necrosis factor alpha (TNF-alpha), 
      interleukin-4 (IL-4), and IL-8 levels. In general, we found increases in the 
      percentages of IL-4-, gamma-IFN-, and TNF-alpha-producing lymphocytes in the 
      infected piglets compared to the percentages in the uninfected control animals, 
      while there was a decrease in the percentage of IL-8-producing monocytes. We 
      believe that these findings reflect a general lymphocyte activation stage that is 
      created due to the infection and that occurs in combination with impairment of 
      the monocyte function, possibly due to the ongoing viral replication in these 
      cells. Single-cell bronchial lymph node preparations exhibited very much the same 
      cytokine profiles as peripheral blood mononuclear cells except for a lack of IL-8 
      production. When the levels of the individual cytokines in the three groups of 
      PRRSV-infected piglets were compared, the levels of cytokine expression at 4 
      weeks diverged from those at 2 and 6 weeks, in that there was a significant 
      decrease in the numbers of lymphocytes producing gamma-IFN and TNF-alpha. This 
      tendency was also observed among blood monocytes and lymph node macrophages. 
      Possible reasons for this temporary immunosuppression in the piglets at 4 weeks 
      are discussed.
FAU - Aasted, B
AU  - Aasted B
AD  - The Royal Veterinary and Agricultural University, DK-1870 Frederiksberg C, 
      Kalvehave DK-4771, Denmark. bas@kvl.dk
FAU - Bach, P
AU  - Bach P
FAU - Nielsen, J
AU  - Nielsen J
FAU - Lind, P
AU  - Lind P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Clin Diagn Lab Immunol
JT  - Clinical and diagnostic laboratory immunology
JID - 9421292
RN  - 0 (Cytokines)
RN  - 56092-81-0 (Ionomycin)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Animals
MH  - Cytokines/*biosynthesis
MH  - Female
MH  - Fetal Diseases/*immunology
MH  - Infectious Disease Transmission, Vertical
MH  - Ionomycin/pharmacology
MH  - Leukocytes, Mononuclear/*immunology
MH  - Lymph Nodes/*immunology
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology
MH  - Pregnancy
MH  - Swine
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Uterus/virology
PMC - PMC130102
EDAT- 2002/11/05 04:00
MHDA- 2003/04/16 05:00
PMCR- 2002/11/01
CRDT- 2002/11/05 04:00
PHST- 2002/11/05 04:00 [pubmed]
PHST- 2003/04/16 05:00 [medline]
PHST- 2002/11/05 04:00 [entrez]
PHST- 2002/11/01 00:00 [pmc-release]
AID - 0066 [pii]
AID - 10.1128/cdli.9.6.1229-1234.2002 [doi]
PST - ppublish
SO  - Clin Diagn Lab Immunol. 2002 Nov;9(6):1229-34. doi: 
      10.1128/cdli.9.6.1229-1234.2002.