PMID- 12414770
OWN - NLM
STAT- MEDLINE
DCOM- 20030415
LR  - 20190508
IS  - 1071-412X (Print)
IS  - 1098-6588 (Electronic)
IS  - 1071-412X (Linking)
VI  - 9
IP  - 6
DP  - 2002 Nov
TI  - Comparative study of the presence of Chlamydia pneumoniae in cerebrospinal fluid 
      of Patients with clinically definite and monosymptomatic multiple sclerosis.
PG  - 1332-7
AB  - There is considerable controversy concerning the evidence for the presence of 
      Chlamydia pneumoniae in the cerebrospinal fluid (CSF) of both multiple sclerosis 
      (MS) patients and patients with other neurological diseases (OND). In order to 
      clarify this issue, the laboratories at Vanderbilt University Medical Center 
      (VUMC) and the University of South Florida (USF) examined the reproducibility of 
      their respective PCR assays for the detection of C. pneumoniae DNA in the CSF of 
      a common group of MS patients and OND controls. The two laboratories used 
      different DNA extraction and PCR techniques in order to determine the prevalence 
      of the C. pneumoniae genome in both monosymptomatic and clinically definite MS 
      patients as well as in OND controls. In clinically definite MS patients, the VUMC 
      and USF detection rates were 72 and 61%, respectively, and in patients with 
      monosymptomatic MS, the VUMC and USF detection rates were 41 and 54%, 
      respectively. The PCR signal was positive for 7% of the OND controls at VUMC and 
      for 16% at USF. These studies confirm our previous reports concerning the high 
      prevalence of C. pneumoniae in the CSF of MS patients. The presence of C. 
      pneumoniae in patients with monosymptomatic MS would also suggest that infection 
      with the organism occurs early in the course of the disease.
FAU - Sriram, Subramaniam
AU  - Sriram S
AD  - Department of Neurology. Department of Pathology, Vanderbilt School of Medicine, 
      Nashville, Tennessee, USA. Srirams@ctrvax.vanderbilt.edu
FAU - Yao, Song-Yi
AU  - Yao SY
FAU - Stratton, Charles
AU  - Stratton C
FAU - Calabresi, Peter
AU  - Calabresi P
FAU - Mitchell, William
AU  - Mitchell W
FAU - Ikejima, Hideaki
AU  - Ikejima H
FAU - Yamamoto, Yoshimasa
AU  - Yamamoto Y
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Clin Diagn Lab Immunol
JT  - Clinical and diagnostic laboratory immunology
JID - 9421292
RN  - 0 (Bacterial Outer Membrane Proteins)
RN  - 0 (major outer membrane protein, Chlamydia pneumoniae)
SB  - IM
CIN - Clin Diagn Lab Immunol. 2003 Sep;10(5):977-8; author reply 978. PMID: 12965938
MH  - Adult
MH  - Aged
MH  - Bacterial Outer Membrane Proteins/genetics
MH  - Cerebrospinal Fluid/*microbiology
MH  - Chlamydophila pneumoniae/*isolation & purification
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Multiple Sclerosis/cerebrospinal fluid/*microbiology
MH  - Polymerase Chain Reaction
PMC - PMC130123
EDAT- 2002/11/05 04:00
MHDA- 2003/04/16 05:00
PMCR- 2002/11/01
CRDT- 2002/11/05 04:00
PHST- 2002/11/05 04:00 [pubmed]
PHST- 2003/04/16 05:00 [medline]
PHST- 2002/11/05 04:00 [entrez]
PHST- 2002/11/01 00:00 [pmc-release]
AID - 0156 [pii]
AID - 10.1128/cdli.9.6.1332-1337.2002 [doi]
PST - ppublish
SO  - Clin Diagn Lab Immunol. 2002 Nov;9(6):1332-7. doi: 
      10.1128/cdli.9.6.1332-1337.2002.