PMID- 12414800
OWN - NLM
STAT- MEDLINE
DCOM- 20030210
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 278
IP  - 1
DP  - 2003 Jan 3
TI  - Resolution of the V1 ATPase from Manduca sexta into subcomplexes and 
      visualization of an ATPase-active A3B3EG complex by electron microscopy.
PG  - 270-5
AB  - The effect of the ATPase activity of Manduca sexta V(1) ATPase by the amphipathic 
      detergent lauryldimethylamine oxide (LDAO) and the relationship of these 
      activities to the subunit composition of V(1) were studied. The V(1) was highly 
      activated in the presence of 0.04-0.06% LDAO combined with release of the 
      subunits H, C, and F from the enzyme. Increase of LDAO concentration to 0.1-0.2% 
      caused the characterized subcomplexes A(3)B(3)HEGF and A(3)B(3)EG with a 
      remaining ATPase activity of 52 and 65%, respectively. The hydrolytic-active 
      A(3)B(3)EG subcomplex has been visualized by electron microscopy showing six 
      major masses of density in a pseudo-hexagonal arrangement surrounding a seventh 
      mass. The compositions of the various subcomplexes and fragments of V(1) provide 
      an organization of the subunits in the enzyme in the framework of the known 
      three-dimensional reconstruction of the V(1) ATPase from M. sexta (Radermacher, 
      M., Ruiz, T., Wieczorek, H., and Gruber, G. (2001) J. Struct. Biol. 135, 26-37).
FAU - Rizzo, Vincenzo F
AU  - Rizzo VF
AD  - Universitat des Saarlandes, Fachrichtung 2.5-Biophysik, D-66421 Homburg, Germany.
FAU - Coskun, Unal
AU  - Coskun U
FAU - Radermacher, Michael
AU  - Radermacher M
FAU - Ruiz, Teresa
AU  - Ruiz T
FAU - Armbruster, Andrea
AU  - Armbruster A
FAU - Gruber, Gerhard
AU  - Gruber G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20021031
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Detergents)
RN  - 0 (Dimethylamines)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Protein Subunits)
RN  - 4F6FC4MI8W (dodecyldimethylamine oxide)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 3.6.1.- (Vacuolar Proton-Translocating ATPases)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - Animals
MH  - Detergents/chemistry
MH  - Dimethylamines/chemistry
MH  - Macromolecular Substances
MH  - Manduca/*enzymology
MH  - Microscopy, Electron
MH  - Models, Molecular
MH  - Protein Structure, Quaternary
MH  - Protein Subunits/chemistry/metabolism
MH  - Structure-Activity Relationship
MH  - Vacuolar Proton-Translocating ATPases/*chemistry/metabolism/ultrastructure
EDAT- 2002/11/05 04:00
MHDA- 2003/02/11 04:00
CRDT- 2002/11/05 04:00
PHST- 2002/11/05 04:00 [pubmed]
PHST- 2003/02/11 04:00 [medline]
PHST- 2002/11/05 04:00 [entrez]
AID - S0021-9258(19)31268-2 [pii]
AID - 10.1074/jbc.M208623200 [doi]
PST - ppublish
SO  - J Biol Chem. 2003 Jan 3;278(1):270-5. doi: 10.1074/jbc.M208623200. Epub 2002 Oct 
      31.