PMID- 12414925
OWN - NLM
STAT- MEDLINE
DCOM- 20021213
LR  - 20240410
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 76
IP  - 23
DP  - 2002 Dec
TI  - Capture and transfer of simian immunodeficiency virus by macaque dendritic cells 
      is enhanced by DC-SIGN.
PG  - 11827-36
AB  - Dendritic cells (DCs) are among the first cells encountered by human and simian 
      immunodeficiency virus (HIV and SIV) following mucosal infection. Because these 
      cells efficiently capture and transmit virus to T cells, they may play a major 
      role in mediating HIV and SIV infection. Recently, a C-type lectin protein 
      present on DCs, DC-specific ICAM-3-grabbing nonintegrin (DC-SIGN), was shown to 
      efficiently bind and present HIV and SIV to CD4(+), coreceptor-positive cells in 
      trans. However, the significance of DC-SIGN for virus transmission and 
      pathogenesis in vivo remains unclear. Because SIV infection of macaques may 
      represent the best model to study the importance of DC-SIGN in HIV infection, we 
      cloned and characterized pig-tailed macaque DC-SIGN and generated monoclonal 
      antibodies (MAbs) against it. We demonstrate that, like human DC-SIGN, pig-tailed 
      macaque DC-SIGN (ptDC-SIGN) is expressed on DCs and macrophages but not on 
      monocytes, T cells, or B cells. Moderate levels of ptDC-SIGN expression were 
      detected on the surface of DCs, and low-level expression was found on 
      macrophages. Additionally, we show that ptDC-SIGN efficiently binds and transmits 
      replication-competent SIVmne variants to CD4(+), coreceptor-positive cells. 
      Moreover, transmission of virus between pig-tailed macaque DCs and CD4(+) T cells 
      is largely ptDC-SIGN dependent. Interestingly, MAbs directed against ptDC-SIGN 
      vary in the capacity to block transmission of different SIVmne variants. These 
      data demonstrate that ptDC-SIGN plays a central role in transmitting virus from 
      macaque DCs to T cells, and they suggest that SIVmne variants may differ in their 
      interactions with ptDC-SIGN. Thus, SIVmne infection of pig-tailed macaques may 
      provide an opportunity to investigate the significance of DC-SIGN in primate 
      lentiviral infections.
FAU - Yu Kimata, Monica T
AU  - Yu Kimata MT
AD  - Department of Virology and Immunology, Southwest Foundation for Biomedical 
      Research, San Antonio, Texas 78227, USA.
FAU - Cella, Marina
AU  - Cella M
FAU - Biggins, Julia E
AU  - Biggins JE
FAU - Rorex, Colin
AU  - Rorex C
FAU - White, Robert
AU  - White R
FAU - Hicks, Sarah
AU  - Hicks S
FAU - Wilson, Joelle M
AU  - Wilson JM
FAU - Patel, Parul G
AU  - Patel PG
FAU - Allan, Jonathan S
AU  - Allan JS
FAU - Colonna, Marco
AU  - Colonna M
FAU - Kimata, Jason T
AU  - Kimata JT
LA  - eng
GR  - R56 AI047725/AI/NIAID NIH HHS/United States
GR  - T32 AI007522/AI/NIAID NIH HHS/United States
GR  - AI07522/AI/NIAID NIH HHS/United States
GR  - R01 AI047725/AI/NIAID NIH HHS/United States
GR  - AI47725/AI/NIAID NIH HHS/United States
GR  - AI49803/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (DC-specific ICAM-3 grabbing nonintegrin)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Receptors, Cell Surface)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/pharmacology
MH  - Binding Sites
MH  - CD4-Positive T-Lymphocytes/virology
MH  - Cell Adhesion Molecules/antagonists & inhibitors/chemistry/genetics/*physiology
MH  - Cell Line
MH  - Dendritic Cells/*immunology/*virology
MH  - HIV-1/immunology/pathogenicity
MH  - Humans
MH  - Lectins, C-Type/antagonists & inhibitors/chemistry/genetics/*physiology
MH  - Macaca nemestrina
MH  - Receptors, Cell Surface/antagonists & inhibitors/chemistry/genetics/*physiology
MH  - Sequence Deletion
MH  - Simian Acquired Immunodeficiency Syndrome/etiology
MH  - Simian Immunodeficiency Virus/*immunology/*pathogenicity
PMC - PMC136877
EDAT- 2002/11/05 04:00
MHDA- 2002/12/17 04:00
PMCR- 2002/12/01
CRDT- 2002/11/05 04:00
PHST- 2002/11/05 04:00 [pubmed]
PHST- 2002/12/17 04:00 [medline]
PHST- 2002/11/05 04:00 [entrez]
PHST- 2002/12/01 00:00 [pmc-release]
AID - 0882 [pii]
AID - 10.1128/jvi.76.23.11827-11836.2002 [doi]
PST - ppublish
SO  - J Virol. 2002 Dec;76(23):11827-36. doi: 10.1128/jvi.76.23.11827-11836.2002.