PMID- 12415568
OWN - NLM
STAT- MEDLINE
DCOM- 20030528
LR  - 20171116
IS  - 0263-6484 (Print)
IS  - 0263-6484 (Linking)
VI  - 20
IP  - 4
DP  - 2002 Dec
TI  - Interleukin-18 enhances HIV-1 production in a human chronically-infected T cell 
      line (H9-V).
PG  - 333-7
AB  - Interleukin-18 (IL-18) is a recently identified immunoregulatory cytokine 
      expressed by activated macrophages, that induces production of interferon-gamma 
      (IFN-gamma) and Th-1 development. Recently some investigators reported 
      controversial in vitro data on IL-18 stimulation of HIV-1 replication in several 
      cell lines. In the present study the effect of IL-18 on HIV replication in a 
      human chronically HIV-1-infected lymphocytic T cell line (H9-V) was investigated. 
      HIV-1 replication was determined by an immunoassay method in order to evaluate 
      the content of p24 antigen in the cell culture supernatants. Stimulation of H9-V 
      cells with IL-18 resulted in increased production of p24, especially at 
      concentrations of 0.01 microg ml(-1) and 0.10 microg ml(-1). Moreover a 
      significant and persistent IL-18 stimulation of HIV-1 replication was observed at 
      a concentration of 0.01 microg ml(-1) during a 7-day period. Pre-treatment of 
      IL-18 with a specific neutralizing monoclonal antibody significantly reduced 
      HIV-1 replication. These experiments show that IL-18 promotes the increase of 
      HIV-1 replication in human chronically-infected lymphocytic T cells and confirm 
      the role of IL-18 as a proimflammatory cytokine in stimulating and maintaining 
      HIV-1 replication during the course of the disease. In a successive set of 
      experiments, since one of the main activities of IL-18 is the induction of 
      IFN-gamma, we evaluated the effect of this biological modifier on H9-V cells. In 
      particular, IFN-gamma shows a significant effect on cell replication and on 
      reduction of CD4 and CD71 surface expression.
CI  - Copyright 2002 John Wiley & Sons, Ltd.
FAU - Pugliese, Agostino
AU  - Pugliese A
AD  - Department of Medical and Surgical Sciences, Section of Clinical Microbiology, 
      Amedeo di Savoia Hospital, University of Turin, Corso Svizzera 164-20249 Turin, 
      Italy. pugliese@dealer.it
FAU - Gennero, Luisa
AU  - Gennero L
FAU - Vidotto, Valerio
AU  - Vidotto V
FAU - Speranza, Filippo
AU  - Speranza F
FAU - Tambini, Roberto
AU  - Tambini R
FAU - Torre, Donato
AU  - Torre D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cell Biochem Funct
JT  - Cell biochemistry and function
JID - 8305874
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, B-Lymphocyte)
RN  - 0 (CD4 Antigens)
RN  - 0 (CD71 antigen)
RN  - 0 (HIV Core Protein p24)
RN  - 0 (Interleukin-18)
RN  - 0 (Receptors, Transferrin)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Antigens, CD/biosynthesis
MH  - Antigens, Differentiation, B-Lymphocyte/biosynthesis
MH  - CD4 Antigens/biosynthesis
MH  - Cell Line
MH  - Cell Membrane/metabolism
MH  - Cell Separation
MH  - Flow Cytometry
MH  - HIV Core Protein p24/metabolism
MH  - HIV-1/*metabolism
MH  - Humans
MH  - Immunoassay
MH  - Interferon-gamma/metabolism
MH  - Interleukin-18/*pharmacology
MH  - Receptors, Transferrin
MH  - T-Lymphocytes/*virology
EDAT- 2002/11/05 04:00
MHDA- 2003/05/29 05:00
CRDT- 2002/11/05 04:00
PHST- 2002/11/05 04:00 [pubmed]
PHST- 2003/05/29 05:00 [medline]
PHST- 2002/11/05 04:00 [entrez]
AID - 10.1002/cbf.981 [doi]
PST - ppublish
SO  - Cell Biochem Funct. 2002 Dec;20(4):333-7. doi: 10.1002/cbf.981.