PMID- 12417058
OWN - NLM
STAT- MEDLINE
DCOM- 20030304
LR  - 20200225
IS  - 1582-1838 (Print)
IS  - 1582-4934 (Electronic)
IS  - 1582-1838 (Linking)
VI  - 6
IP  - 3
DP  - 2002 Jul-Sep
TI  - Co-stimulatory and adhesion molecules of dendritic cells in rheumatoid arthritis.
PG  - 415-25
AB  - Dendritic cells (DCs) in the rheumatoid arthritis (RA) joint mediate the 
      immunopathological process and act as a potent antigen presenting cell. We 
      compared the expression of co-stimulatory and adhesion molecules on DCs in RA 
      patients versus controls with traumatic joint lesions and evaluated the 
      correlation between the immunophenotypical presentation of DCs and the clinical 
      status of the disease. Samples of peripheral venous blood, synovial fluid (SF) 
      and synovial tissue (ST) were obtained from 10 patients with RA at the time of 
      hip or knee replacement and from 9 control patients with knee arthroscopy for 
      traumatic lesions. Clinical status was appreciated using the DAS28 score. Blood, 
      SF and dissociated ST cell populations were separated by centrifugation and 
      analyzed by flow cytometry. Cells phenotypes were identified using three-color 
      flow cytometry analysis for the following receptors HLA-DR, CD80, CD83, CD86, 
      CD11c, CD18, CD54, CD58, CD3, CD4, CD8, CD19, CD20, CD14, CD16, CD56. HLA-DR 
      molecules, co-stimulatory receptors CD80, CD86, CD83 and adhesion molecules CD18, 
      CD11c, CD54, CD58, were analyzed by two-color immunofluorescence microscopy on ST 
      serial sections. In patients with active RA (DAS28>5.1) we found a highly 
      differentiated subpopulation of DCs in the ST and SF that expressed an activated 
      phenotype (HLA-DR, CD86+, CD80+, CD83+, CD11c+, CD54+, CD58+). No differences 
      were found between circulating DCs from RA patients and control patients. Our 
      data suggest an interrelationship between clinical outcome and the 
      immunophenotypical presentation of DCs. Clinical active RA (DAS28>5.1) is 
      associated with high incidence of activated DCs population in the ST and SF as 
      demonstrated by expression of adhesion and co-stimulatory molecules.
FAU - Balanescu, Andra
AU  - Balanescu A
AD  - Dept. of Rheumatology and Internal Medicine, St Maria Hospital, Carol Davila 
      University of Medicine and Pharmacy, Bucharest, Romania. 
      balanescuandra@hotmail.com
FAU - Radu, E
AU  - Radu E
FAU - Nat, Roxana
AU  - Nat R
FAU - Regalia, T
AU  - Regalia T
FAU - Bojinca, Violeta
AU  - Bojinca V
FAU - Predescu, V
AU  - Predescu V
FAU - Predeteanu, Denisa
AU  - Predeteanu D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Cell Mol Med
JT  - Journal of cellular and molecular medicine
JID - 101083777
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation)
RN  - 0 (Cell Adhesion Molecules)
SB  - IM
MH  - Adult
MH  - Antigens, CD/*metabolism
MH  - Antigens, Differentiation
MH  - Arthritis, Rheumatoid/*immunology/pathology
MH  - Arthroplasty, Replacement, Hip
MH  - Arthroplasty, Replacement, Knee
MH  - Cell Adhesion Molecules/*metabolism
MH  - Cell Differentiation
MH  - Dendritic Cells/immunology/*metabolism
MH  - Female
MH  - Flow Cytometry
MH  - Hip Joint/immunology/pathology
MH  - Humans
MH  - Knee Joint/immunology/pathology
MH  - Male
MH  - Middle Aged
MH  - Romania
MH  - Synovial Fluid/cytology/immunology
PMC - PMC6740068
EDAT- 2002/11/06 04:00
MHDA- 2003/03/05 04:00
PMCR- 2002/07/01
CRDT- 2002/11/06 04:00
PHST- 2002/11/06 04:00 [pubmed]
PHST- 2003/03/05 04:00 [medline]
PHST- 2002/11/06 04:00 [entrez]
PHST- 2002/07/01 00:00 [pmc-release]
AID - 006.003.10 [pii]
AID - JCMM415 [pii]
AID - 10.1111/j.1582-4934.2002.tb00520.x [doi]
PST - ppublish
SO  - J Cell Mol Med. 2002 Jul-Sep;6(3):415-25. doi: 
      10.1111/j.1582-4934.2002.tb00520.x.