PMID- 12417794
OWN - NLM
STAT- MEDLINE
DCOM- 20021210
LR  - 20171101
IS  - 0030-2414 (Print)
IS  - 0030-2414 (Linking)
VI  - 63
IP  - 4
DP  - 2002
TI  - Investigations for fine mapping of amplifications in chromosome 3q26.3-28 
      frequently occurring in squamous cell carcinomas of the head and neck.
PG  - 385-92
AB  - OBJECTIVE: Overrepresentations of chromosomal material on the long arm of 
      chromosome 3 frequently occur in squamous cell carcinoma of the head and neck. 
      This experimental study was conducted for further fine mapping of these 
      overrepresentations by interphase fluorescence in situ hybridization (FISH) of 
      tumor cells in cell lines. METHODS: Seven cell lines derived from squamous cell 
      carcinomas of the head and neck were investigated by comparative genomic 
      hybridization to analyze unbalanced chromosomal aberrations. Overrepresentations 
      of chromosomal material on the telomeric part of the long arm of chromosome 3 
      were further analyzed by interphase FISH using YAC contig clones. RESULTS: 
      Chromosomal aberrations which frequently occurred were overrepresentations on 5p 
      (n = 4), 7p (n = 5), 11q13 (n = 3), 15q (n = 5), 17q (n = 3), 19q (n = 2), 20q (n 
      = 2) and 22q (n = 3). Reoccurring losses of chromosomal material were found in 3p 
      (n = 3), 7q (n = 2), 18q (n = 3) and 19p (n = 2). Gains of chromosomal material 
      on chromosome 3q were found in 4 out of 7 cell lines, with a high copy number of 
      amplifications occurring in the chromosomal region of 3q26.3-28. Further 
      experiments revealed a physical mapping of this amplification to a narrow band of 
      13.8 Mbp on chromosome 3q, whose amplification borders were represented by the 
      YAC clones 754_f_3 centomeric and 955_b_2 telomeric. CONCLUSIONS: By FISH, the 
      amplification of chromosomal material on 3q could be fine mapped on a narrow band 
      on 3q26.3-27. This aberration can be considered as a breakpoint in tumorigenesis. 
      Putative candidate oncogenes and tumor suppressor genes located in this region 
      might be a target for mutations leading to tumor progression.
CI  - Copyright 2002 S. Karger AG, Basel
FAU - Riazimand, Seyed H
AU  - Riazimand SH
AD  - Department of Otorhinolaryngology, School of Medicine, University of Mainz, 
      Germany.
FAU - Welkoborsky, Hans-J
AU  - Welkoborsky HJ
FAU - Bernauer, Hubert S
AU  - Bernauer HS
FAU - Jacob, Roland
AU  - Jacob R
FAU - Mann, Wolf J
AU  - Mann WJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Oncology
JT  - Oncology
JID - 0135054
SB  - IM
MH  - Carcinoma, Squamous Cell/*genetics/pathology
MH  - *Chromosome Aberrations
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 3
MH  - Gene Amplification
MH  - Head and Neck Neoplasms/*genetics/pathology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Tumor Cells, Cultured
EDAT- 2002/11/06 04:00
MHDA- 2002/12/11 04:00
CRDT- 2002/11/06 04:00
PHST- 2002/11/06 04:00 [pubmed]
PHST- 2002/12/11 04:00 [medline]
PHST- 2002/11/06 04:00 [entrez]
AID - 66220 [pii]
AID - 10.1159/000066220 [doi]
PST - ppublish
SO  - Oncology. 2002;63(4):385-92. doi: 10.1159/000066220.