PMID- 1241944
OWN - NLM
STAT- MEDLINE
DCOM- 19770103
LR  - 20111117
IS  - 0014-2980 (Print)
IS  - 0014-2980 (Linking)
VI  - 5
IP  - 2
DP  - 1975 Feb
TI  - Radioactive antigen suicide of an anti-DNP (2,4-dinitrophenyl) clone. II. 
      Follow-up of clones relatively resistant to radioactive antigen suicide when 
      initially selected.
PG  - 131-9
AB  - After suicide of the anti-dinitrophenyl (DNP) clone E21 in a previous experiment 
      (Eur. J. Immunol. 1975. 5:58) several new clones (S clones) appeared promptly in 
      the recipients. There was compelling evidence that they were then resistant to 
      suicide, relative to E21. We now report further studies on these S clones and 
      their antibodies. The antibodies of half of the S clones had affinities ten to 
      one hundred times higher than E21 (3 x 10(-7) M); none was lower. The four tested 
      were also able to bind DNP on the particular conjugate used for the suicide 
      procedure. Three of the S clones were serially propagated, they showed a very 
      great capacity for proliferation, transferring into 510, 85 and 110 recipients 
      each. When their cells were tested at subsequent passages, they recovered early 
      after inhibition by radioactive antigen. Two S clones may still have been 
      somewhat refractory to suicide but, probably in all three, rapid proliferation 
      was largely responsible for the recovery of the few cells escaping suicide, and 
      helped them to appear suicide resistant.
FAU - Willcox, H N
AU  - Willcox HN
FAU - McMichael, A J
AU  - McMichael AJ
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (Dinitrobenzenes)
RN  - 0 (Haptens)
RN  - 0 (Iodine Radioisotopes)
RN  - 0 (Nitrobenzenes)
SB  - IM
MH  - Age Factors
MH  - Animals
MH  - *Antibody Formation
MH  - Antibody-Producing Cells/*radiation effects
MH  - Binding Sites, Antibody
MH  - Cell Division
MH  - Clone Cells/*immunology
MH  - Dinitrobenzenes/*immunology
MH  - Female
MH  - Haptens
MH  - Immune Tolerance
MH  - Iodine Radioisotopes
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Nitrobenzenes/*immunology
MH  - Spleen/immunology
EDAT- 1975/02/01 00:00
MHDA- 1975/02/01 00:01
CRDT- 1975/02/01 00:00
PHST- 1975/02/01 00:00 [pubmed]
PHST- 1975/02/01 00:01 [medline]
PHST- 1975/02/01 00:00 [entrez]
AID - 10.1002/eji.1830050212 [doi]
PST - ppublish
SO  - Eur J Immunol. 1975 Feb;5(2):131-9. doi: 10.1002/eji.1830050212.