PMID- 12419493 OWN - NLM STAT- MEDLINE DCOM- 20021230 LR - 20190701 IS - 0304-3940 (Print) IS - 0304-3940 (Linking) VI - 333 IP - 2 DP - 2002 Nov 22 TI - Developmental exposure to the antiretroviral drug zidovudine increases brain levels of brain-derived neurotrophic factor in mice. PG - 111-4 AB - We analyzed brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) protein content in the central nervous system (CNS) of developing and adult mice exposed to the antiretroviral agent zidovudine (AZT) during prenatal and early postnatal period. Pregnant CD-1 mice received per os twice daily AZT (160 mg/kg) or vehicle from gestation day 10 until lactation day 7. BDNF and NGF contents were measured by enzyme immunoassays in male and female offspring on day 7, 21 or 60. In AZT-exposed females, BDNF levels were significantly increased in the hippocampus (days 7 and 21), in the cortex (day 60) and in the hypothalamus (day 21), while in males AZT exposure increased BDNF in the cortex on day 21. These findings support the hypothesis that developmental AZT exposure interferes with CNS development. FAU - Calamandrei, Gemma AU - Calamandrei G AD - Section of Comparative Psychology, Istituto Superiore di Sanita, Viale Regina Elena 299, I-00161, Roma, Italy. gemma.calamandrei@iss.it FAU - Valanzano, Angela AU - Valanzano A FAU - Puopolo, Maria AU - Puopolo M FAU - Aloe, Luigi AU - Aloe L LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Ireland TA - Neurosci Lett JT - Neuroscience letters JID - 7600130 RN - 0 (Antimetabolites) RN - 0 (Brain-Derived Neurotrophic Factor) RN - 4B9XT59T7S (Zidovudine) RN - 9061-61-4 (Nerve Growth Factor) SB - IM MH - Age Factors MH - Animals MH - Animals, Newborn MH - Antimetabolites/*pharmacology MH - Brain/*drug effects/growth & development/metabolism MH - Brain-Derived Neurotrophic Factor/*drug effects/metabolism MH - Female MH - Male MH - Mice MH - Nerve Growth Factor/drug effects/metabolism MH - Pregnancy MH - *Prenatal Exposure Delayed Effects MH - Sex Factors MH - Time Factors MH - Zidovudine/*pharmacology EDAT- 2002/11/07 04:00 MHDA- 2002/12/31 04:00 CRDT- 2002/11/07 04:00 PHST- 2002/11/07 04:00 [pubmed] PHST- 2002/12/31 04:00 [medline] PHST- 2002/11/07 04:00 [entrez] AID - S0304394002010236 [pii] AID - 10.1016/s0304-3940(02)01023-6 [doi] PST - ppublish SO - Neurosci Lett. 2002 Nov 22;333(2):111-4. doi: 10.1016/s0304-3940(02)01023-6.