PMID- 12419587
OWN - NLM
STAT- MEDLINE
DCOM- 20021120
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 138
IP  - 1
DP  - 2002 Oct 1
TI  - Combined analysis of morphology and fluorescence in situ hybridization in 
      follow-up of minimal residual disease in a child with Philadelphia-positive acute 
      lymphoblastic leukemia.
PG  - 64-8
AB  - The past decade has brought new technologies to the study of minimal residual 
      disease (MRD) in leukemia. Each of them has limitations and is far from being 
      accurate. Recently, a new multiparametric cell scanning system (Duet) was 
      introduced to the field of MRD detection. This system has the advantage of 
      automatically scanning large numbers of cells and performing combined analysis of 
      morphology and fluorescence in situ hybridization (FISH) on the same cell. We 
      used this system to characterize the lineage and degree of maturation of the 
      cells carrying the minor m-BCR/ABL fusion, in a follow-up of an 8-year-old boy 
      with Philadelphia-positive (Ph(+)) acute lymphoblastic leukemia (ALL). The boy 
      was treated using a high-risk protocol and was closely monitored with FISH 
      analysis for cells carrying the m-BCR/ABL fusion. Consecutive analysis along 2.5 
      years from remission showed 0.2-4.5% m-BCR/ALB(+) cells in the peripheral blood 
      (PB), which is within the accepted background range for this method. The combined 
      analysis found that all the m-BCR/ABL(+) cells were mature lymphocytes. Because 
      mature lymphocytes have a long life span in the circulation, this finding 
      supports the fact that the patient is in remission. Moreover, since mature 
      differentiated cells have a low proliferative capacity, there is a low risk for 
      relapse.
FAU - Bielorai, Bella
AU  - Bielorai B
AD  - Department of Pediatric Hemato-Oncology and Institute of Hematology, The Chaim 
      Sheba Medical Center, Tel-Hashomer and Sackler School of Medicine, Tel-Aviv 
      University, 5262, Tel-Aviv, Israel.
FAU - Golan, Hana
AU  - Golan H
FAU - Trakhtenbrot, Luba
AU  - Trakhtenbrot L
FAU - Reichart, Malka
AU  - Reichart M
FAU - Toren, Amos
AU  - Toren A
FAU - Daniely, Michal
AU  - Daniely M
FAU - Zilberstein, Yulia
AU  - Zilberstein Y
FAU - Amariglio, Ninette
AU  - Amariglio N
FAU - Rechavi, Gideon
AU  - Rechavi G
FAU - Kaplinsky, Chaim
AU  - Kaplinsky C
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Cell Division
MH  - Cell Lineage
MH  - Cell Size
MH  - Child
MH  - Child, Preschool
MH  - Follow-Up Studies
MH  - Fusion Proteins, bcr-abl/analysis
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*genetics/*pathology
MH  - Male
MH  - Neoplasm, Residual/*genetics/*pathology
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics/*pathology
EDAT- 2002/11/07 04:00
MHDA- 2002/11/26 04:00
CRDT- 2002/11/07 04:00
PHST- 2002/11/07 04:00 [pubmed]
PHST- 2002/11/26 04:00 [medline]
PHST- 2002/11/07 04:00 [entrez]
AID - S0165460802005782 [pii]
AID - 10.1016/s0165-4608(02)00578-2 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2002 Oct 1;138(1):64-8. doi: 
      10.1016/s0165-4608(02)00578-2.