PMID- 12419851
OWN - NLM
STAT- MEDLINE
DCOM- 20030115
LR  - 20181113
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 99
IP  - 23
DP  - 2002 Nov 12
TI  - Transcriptional profiles associated with aging and middle age-onset caloric 
      restriction in mouse hearts.
PG  - 14988-93
AB  - To provide a global analysis of gene expression in the aging heart, we monitored 
      the expression of 9,977 genes simultaneously in 5- and 30-month-old male B6C3F(1) 
      mice by using high-density oligonucleotide microarrays and several statistical 
      techniques. Aging was associated with transcriptional alterations consistent with 
      a metabolic shift from fatty acid to carbohydrate metabolism, increased 
      expression of extracellular matrix genes, and reduced protein synthesis. Caloric 
      restriction (CR) started at 14 months of age resulted in a 19% global inhibition 
      of age-related changes in gene expression. Interestingly, CR also resulted in 
      alterations in gene expression consistent with preserved fatty acid metabolism, 
      reduced endogenous DNA damage, decreased innate immune activity, apoptosis 
      modulation, and a marked cytoskeletal reorganization. These observations provide 
      evidence that aging of the heart is associated with specific transcriptional 
      alterations, and that CR initiated in middle age may retard heart aging by 
      inducing a profound transcriptional reprogramming.
FAU - Lee, Cheol-Koo
AU  - Lee CK
AD  - Molecular and Environmental Toxicology Center, Madison, WI 53706, USA.
FAU - Allison, David B
AU  - Allison DB
FAU - Brand, Jaap
AU  - Brand J
FAU - Weindruch, Richard
AU  - Weindruch R
FAU - Prolla, Tomas A
AU  - Prolla TA
LA  - eng
GR  - R01 AG018922/AG/NIA NIH HHS/United States
GR  - R01AG18922/AG/NIA NIH HHS/United States
GR  - U24DK058776/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20021105
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Enzymes)
RN  - 0 (Proteins)
SB  - IM
MH  - Aging/*genetics
MH  - Algorithms
MH  - Animals
MH  - *Diet, Reducing
MH  - Enzymes/genetics
MH  - Gene Expression Regulation, Developmental/*genetics
MH  - Heart/*growth & development
MH  - Male
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Models, Genetic
MH  - Nucleic Acid Hybridization
MH  - Proteins/genetics
MH  - *Transcription, Genetic
PMC - PMC137532
EDAT- 2002/11/07 04:00
MHDA- 2003/01/16 04:00
PMCR- 2003/05/12
CRDT- 2002/11/07 04:00
PHST- 2002/11/07 04:00 [pubmed]
PHST- 2003/01/16 04:00 [medline]
PHST- 2002/11/07 04:00 [entrez]
PHST- 2003/05/12 00:00 [pmc-release]
AID - 232308999 [pii]
AID - pq2302014988 [pii]
AID - 10.1073/pnas.232308999 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):14988-93. doi: 
      10.1073/pnas.232308999. Epub 2002 Nov 5.