PMID- 12420106
OWN - NLM
STAT- MEDLINE
DCOM- 20030716
LR  - 20131121
IS  - 0340-3696 (Print)
IS  - 0340-3696 (Linking)
VI  - 294
IP  - 8
DP  - 2002 Nov
TI  - Upregulation of interleukin-18 expression in mouse primary keratinocytes induced 
      to differentiate by calcium.
PG  - 370-6
AB  - Interleukin-18 (IL-18) is an immunomodulatory cytokine that stimulates 
      interferon-gamma production by T helper cells. Recently, basal keratinocytes have 
      been shown to constitutively express IL-18, and IL-18 expression increases in the 
      suprabasal keratinocytes in psoriatic lesions. In the study reported here we 
      showed that in mouse epidermis, IL-18 immunoreactivity was markedly increased in 
      the granular and cornified layers. To further investigate whether differentiated 
      keratinocytes synthesize more IL-18, we examined the expression of mouse IL-18 in 
      primary mouse keratinocytes induced to differentiate by calcium, an in vitro cell 
      culture system mimicking keratinocyte differentiation in the epidermis. We 
      demonstrated that IL-18 mRNA and protein in cultured keratinocytes were increased 
      by calcium treatment in a time- and dose-dependent manner. The upregulation of 
      IL-18 was associated with an increase in keratinocyte differentiation markers, 
      and was dependent on the synthesis of new RNAs and proteins. However, the IL-18 
      protein in the cytoplasm was predominantly in the precursor form, and no increase 
      in IL-18 activity was detected in the culture medium treated with calcium. 
      Furthermore, blocking the calcium-induced keratinocyte differentiation with 
      protein kinase C inhibitor inhibited the upregulation of IL-18 expression. These 
      findings suggest that IL-18 is synthesized in keratinocytes mainly in the 
      inactive precursor form, and its expression is upregulated as basal keratinocytes 
      differentiate in the epidermis.
FAU - Kong, Juan
AU  - Kong J
AD  - Department of Medicine, University of Chicago, 5841 S. Maryland Avenue, Chicago, 
      IL 60637, USA.
FAU - Li, Yan Chun
AU  - Li YC
LA  - eng
GR  - DK59327/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20021008
PL  - Germany
TA  - Arch Dermatol Res
JT  - Archives of dermatological research
JID - 8000462
RN  - 0 (Interleukin-18)
RN  - 63231-63-0 (RNA)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/*pharmacology
MH  - Cell Differentiation/drug effects
MH  - Cells, Cultured
MH  - Enzyme Activation/drug effects/physiology
MH  - Immunohistochemistry
MH  - Interleukin-18/*metabolism
MH  - Keratinocytes/*cytology/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Protein Kinase C/metabolism
MH  - RNA/biosynthesis
MH  - Skin/metabolism
MH  - Up-Regulation
EDAT- 2002/11/07 04:00
MHDA- 2003/07/17 05:00
CRDT- 2002/11/07 04:00
PHST- 2002/04/24 00:00 [received]
PHST- 2002/07/23 00:00 [revised]
PHST- 2002/08/24 00:00 [accepted]
PHST- 2002/11/07 04:00 [pubmed]
PHST- 2003/07/17 05:00 [medline]
PHST- 2002/11/07 04:00 [entrez]
AID - 10.1007/s00403-002-0348-5 [doi]
PST - ppublish
SO  - Arch Dermatol Res. 2002 Nov;294(8):370-6. doi: 10.1007/s00403-002-0348-5. Epub 
      2002 Oct 8.