PMID- 12420294
OWN - NLM
STAT- MEDLINE
DCOM- 20030616
LR  - 20071115
IS  - 1040-452X (Print)
IS  - 1040-452X (Linking)
VI  - 64
IP  - 1
DP  - 2003 Jan
TI  - Chromosomal location, structure, and temporal expression of the 
      platelet-activating factor receptor (PAFr) gene in porcine endometrium and 
      embryos relative to estrogen receptor alpha gene expression.
PG  - 4-12
AB  - Although platelet-activating factor receptor (PAFr) gene was well characterized 
      in the human, little was known about it in domestic animals. Porcine PAFr gene 
      was mapped using fluorescence in situ hybridization (FISH). The structure of this 
      gene was investigated using a 5' rapid amplification of cDNA ends (RACE) 
      technique. Temporal expression of PAFr and estrogen receptor alpha genes (ER), 
      and distribution of the PAFr transcripts in porcine endometrial and embryonic 
      tissues on days 0, 10, 12, 14, 16, and 18 were analyzed using DNA competitors and 
      reverse transcription and polymerase chain reaction (RT-PCR). The porcine PAFr 
      gene was mapped to SSC6q26-27. Alternative splicing of primary transcripts of the 
      PAFr gene produced two different transcripts. Transcript 1 was expressed in all 
      tissues and cells, and transcript 2 was detected in all tissues but white blood 
      cells. The temporal expression of the PAFr gene in endometrial (P > 0.05) and 
      embryonic (P < 0.05) tissues of pregnant sows increased from day 10 to 16. The 
      temporal expression of ER genes in endometrial tissues of pregnant sows decreased 
      from day 10 to 18 (P < 0.05). In addition, ER expression was detectable in 20-60% 
      of embryonic tissue samples, which generally decreased. In combination with 
      previously obtained data on PAF and estradiol-17beta (E(2)) concentrations in 
      pregnant uterine luminal fluids (pULF), endometrial and embryonic tissues, the 
      present results indicated that the increasing PAFr transcripts were positively 
      associated with increasing levels of PAF. Both ER transcripts and E(2) found in 
      pULF decreased correspondingly from day 13 to 16. These results indicate that via 
      PAFr, PAF could play a dominant role in peri-implantation development in pigs as 
      compared to E(2).
CI  - Copyright 2003 Wiley-Liss, Inc.
FAU - Yang, Wensheng
AU  - Yang W
AD  - Poole Agricultural Center, Department of Animal & Veterinary Sciences, Clemson 
      University, Clemson, South Carolina 29634, USA.
FAU - Diehl, John R
AU  - Diehl JR
FAU - Yerle, Martine
AU  - Yerle M
FAU - Ford, J Joe
AU  - Ford JJ
FAU - Christenson, Ronald K
AU  - Christenson RK
FAU - Roudebush, William E
AU  - Roudebush WE
FAU - Plummer, William E
AU  - Plummer WE
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Mol Reprod Dev
JT  - Molecular reproduction and development
JID - 8903333
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (Platelet Membrane Glycoproteins)
RN  - 0 (Protein Isoforms)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Estrogen)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 0 (platelet activating factor receptor)
SB  - IM
MH  - Animals
MH  - *Chromosome Mapping
MH  - Embryo, Mammalian/*metabolism
MH  - Endometrium/*metabolism
MH  - Estrogen Receptor alpha
MH  - Female
MH  - In Situ Hybridization, Fluorescence
MH  - Platelet Membrane Glycoproteins/biosynthesis/chemistry/*genetics
MH  - Pregnancy
MH  - Protein Isoforms
MH  - RNA, Messenger
MH  - Receptors, Cell Surface/biosynthesis/chemistry/*genetics
MH  - Receptors, Estrogen/biosynthesis/*genetics
MH  - *Receptors, G-Protein-Coupled
MH  - Sequence Analysis, DNA
MH  - Swine
EDAT- 2002/11/07 04:00
MHDA- 2003/06/17 05:00
CRDT- 2002/11/07 04:00
PHST- 2002/11/07 04:00 [pubmed]
PHST- 2003/06/17 05:00 [medline]
PHST- 2002/11/07 04:00 [entrez]
AID - 10.1002/mrd.10217 [doi]
PST - ppublish
SO  - Mol Reprod Dev. 2003 Jan;64(1):4-12. doi: 10.1002/mrd.10217.