PMID- 12421479
OWN - NLM
STAT- MEDLINE
DCOM- 20040802
LR  - 20131121
IS  - 1671-4083 (Print)
IS  - 1671-4083 (Linking)
VI  - 23
IP  - 11
DP  - 2002 Nov
TI  - Protective effects of polypeptide from Chlamys farreri on Hela cells damaged by 
      ultraviolet A.
PG  - 1018-22
AB  - AIM: To study the protective effect of polypeptide isolated from Chlamys farreri 
      (PCF) on Hela cells damaged by ultraviolet A (UVA) in vitro. METHODS: Cell 
      proliferation was determined by MTT method; intra-cellular free calcium [Ca2+]i 
      and rates of apoptosis and death were measured by flow cytometry (FCM). RESULTS: 
      PCF (0.5 %-2 %) enhanced the activities of glutathione peroxidase (GSH-px), 
      superoxide dismutase (SOD), and catalase (CAT), and stimulated cell 
      proliferation. The concentration of [Ca2+]i was increased while the amounts of 
      MDA and the rates of apoptosis and death of the cells were decreased. The 
      differences between the PCF groups and control group were significant (P<0.05, 
      P<0.01). CONCLUSION: PCF protected Hela cells against damage by UVA via its 
      anti-oxidative mechanisms.
FAU - Yao, Ru-Yong
AU  - Yao RY
AD  - Hospital of Medical College, Qingdao University, Qingdao 266003, China.
FAU - Wang, Chun-Bo
AU  - Wang CB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Acta Pharmacol Sin
JT  - Acta pharmacologica Sinica
JID - 100956087
RN  - 0 (Antioxidants)
RN  - 0 (Materia Medica)
RN  - 0 (Peptides)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Antioxidants/isolation & purification/*pharmacology
MH  - *Apoptosis/drug effects/radiation effects
MH  - Calcium/metabolism
MH  - Catalase/metabolism
MH  - Glutathione Peroxidase/metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Materia Medica/isolation & purification/*pharmacology
MH  - Ostreidae/*chemistry
MH  - Peptides/isolation & purification/pharmacology
MH  - Superoxide Dismutase/metabolism
MH  - *Ultraviolet Rays
EDAT- 2002/11/08 04:00
MHDA- 2004/08/03 05:00
CRDT- 2002/11/08 04:00
PHST- 2002/11/08 04:00 [pubmed]
PHST- 2004/08/03 05:00 [medline]
PHST- 2002/11/08 04:00 [entrez]
PST - ppublish
SO  - Acta Pharmacol Sin. 2002 Nov;23(11):1018-22.