PMID- 12421982
OWN - NLM
STAT- MEDLINE
DCOM- 20030114
LR  - 20190515
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 169
IP  - 10
DP  - 2002 Nov 15
TI  - Expression and function of C5a receptor in mouse microvascular endothelial cells.
PG  - 5962-70
AB  - The complement-derived anaphylatoxin, C5a, is a potent phlogistic molecule that 
      mediates its effects by binding to C5a receptor (C5aR; CD88). We now demonstrate 
      specific binding of radiolabeled recombinant mouse C5a to mouse dermal 
      microvascular endothelial cells (MDMEC) with a K(d50) of 3.6 nM and to 
      approximately 15,000-20,000 receptors/cell. Recombinant mC5a competed effectively 
      with binding of [(125)I]rmC5a to MDMEC. Enhanced binding of C5a occurred, as well 
      as increased mRNA for C5aR, after in vitro exposure of MDMEC to LPS, IFN-gamma, 
      or IL-6 in a time- and dose-dependent manner. By confocal microscopy, C5aR could 
      be detected on surfaces of MDMEC using anti-C5aR Ab. In vitro expression of 
      macrophage inflammatory protein-2 (MIP-2) and monocyte chemoattractant protein-1 
      (MCP-1) by MDMEC was also measured. Exposure of MDMEC to C5a or IL-6 did not 
      result in changes in MIP-2 or MCP-1 production, but initial exposure of MDMEC to 
      IL-6, followed by exposure to C5a, resulted in significantly enhanced production 
      of MIP-2 and MCP-1 (but not TNF-alpha and MIP-1alpha). Although LPS or IFN-gamma 
      alone induced some release of MCP-1 and MIP-2, pre-exposure of these monolayers 
      to LPS or IFN-gamma, followed by addition of C5a, resulted in synergistic 
      production of MIP-2 and MCP-1. Following i.v. infusion of LPS into mice, 
      up-regulation of C5aR occurred in the capillary endothelium of mouse lung, as 
      determined by immunostaining. These results support the hypothesis that C5aR 
      expression on MDMEC and on the microvascular endothelium of lung can be 
      up-regulated, suggesting that C5a in the co-presence of additional agonists may 
      mediate pro-inflammatory effects of endothelial cells.
FAU - Laudes, Ines J
AU  - Laudes IJ
AD  - Department of Pathology, University of Michigan Medical School, Ann Arbor, MI 
      48109, USA.
FAU - Chu, Jeffrey C
AU  - Chu JC
FAU - Huber-Lang, Markus
AU  - Huber-Lang M
FAU - Guo, Ren-Feng
AU  - Guo RF
FAU - Riedemann, Niels C
AU  - Riedemann NC
FAU - Sarma, J Vidya
AU  - Sarma JV
FAU - Mahdi, Fakhri
AU  - Mahdi F
FAU - Murphy, Hedwig S
AU  - Murphy HS
FAU - Speyer, Cecilia
AU  - Speyer C
FAU - Lu, Kristina T
AU  - Lu KT
FAU - Lambris, John D
AU  - Lambris JD
FAU - Zetoune, Firas S
AU  - Zetoune FS
FAU - Ward, Peter A
AU  - Ward PA
LA  - eng
GR  - AI 33189/AI/NIAID NIH HHS/United States
GR  - GM 61656/GM/NIGMS NIH HHS/United States
GR  - HL 07517/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Antigens, CD)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CXCL2)
RN  - 0 (Chemokines)
RN  - 0 (Cxcl2 protein, mouse)
RN  - 0 (Interleukin-6)
RN  - 0 (Iodine Radioisotopes)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Peptide Fragments)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptor, Anaphylatoxin C5a)
RN  - 0 (Receptors, Complement)
RN  - 0 (von Willebrand Factor)
RN  - 80295-54-1 (Complement C5a)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adjuvants, Immunologic/pharmacology
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigens, CD/*biosynthesis/immunology/metabolism/*physiology
MH  - Binding, Competitive/immunology
MH  - Cells, Cultured
MH  - Chemokine CCL2/biosynthesis
MH  - Chemokine CXCL2
MH  - Chemokines/biosynthesis
MH  - Complement C5a/*metabolism
MH  - Endothelium, Vascular/cytology/*immunology/*metabolism
MH  - Flow Cytometry
MH  - Fluorescent Antibody Technique, Direct
MH  - Gene Expression Regulation/immunology
MH  - Infusions, Intravenous
MH  - Interferon-gamma/pharmacology
MH  - Interleukin-6/pharmacology
MH  - Iodine Radioisotopes/metabolism
MH  - Lipopolysaccharides/administration & dosage/pharmacology
MH  - Lung/blood supply/immunology/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Inbred DBA
MH  - Microcirculation/cytology/immunology/metabolism
MH  - Microscopy, Confocal
MH  - Molecular Sequence Data
MH  - Peptide Fragments/analysis/metabolism
MH  - RNA, Messenger/biosynthesis
MH  - Receptor, Anaphylatoxin C5a
MH  - Receptors, Complement/*biosynthesis/immunology/metabolism/*physiology
MH  - Up-Regulation/immunology
MH  - von Willebrand Factor/metabolism
EDAT- 2002/11/08 04:00
MHDA- 2003/01/15 04:00
CRDT- 2002/11/08 04:00
PHST- 2002/11/08 04:00 [pubmed]
PHST- 2003/01/15 04:00 [medline]
PHST- 2002/11/08 04:00 [entrez]
AID - 10.4049/jimmunol.169.10.5962 [doi]
PST - ppublish
SO  - J Immunol. 2002 Nov 15;169(10):5962-70. doi: 10.4049/jimmunol.169.10.5962.