PMID- 12423313
OWN - NLM
STAT- MEDLINE
DCOM- 20021213
LR  - 20190513
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 107
IP  - 3
DP  - 2002 Nov
TI  - Generation and characterization of ovine dendritic cells derived from peripheral 
      blood monocytes.
PG  - 366-72
AB  - Dendritic cells (DCs) are professional antigen-presenting cells with a highly 
      immunostimulatory function and the capacity to activate naive T cells. In recent 
      years the rapid progress in mouse and human DC research can be mainly attributed 
      to the generation of DCs from precursor cells in vitro, although a lack of 
      reagents has hampered DC research in many large animal models. Here we describe 
      the generation and characterization of ovine monocyte-derived DCs in vitro. In 
      addition to the characteristic morphology and non-adherence of DCs, peripheral 
      blood mononuclear cell monocytes cultured with ovine granulocyte-macrophage 
      colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) expressed CD11c and 
      major histocompatibility complex (MHC) class II, but did not express CD14. High 
      levels of endocytosis and an ability to stimulate antigen-specific proliferation 
      of CD4 T lymphocytes were also demonstrated.
FAU - Chan, Simon S M
AU  - Chan SS
AD  - Centre for Veterinary Science, Department of Clinical Veterinary Medicine, 
      University of Cambridge, Cambridge, UK.
FAU - McConnell, Ian
AU  - McConnell I
FAU - Blacklaws, Barbara A
AU  - Blacklaws BA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Recombinant Proteins)
RN  - 207137-56-2 (Interleukin-4)
SB  - IM
MH  - Animals
MH  - Antigen Presentation
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - Cell Adhesion/immunology
MH  - Cell Culture Techniques
MH  - Cell Differentiation/immunology
MH  - Dendritic Cells/*immunology
MH  - Endocytosis/immunology
MH  - Female
MH  - Immunophenotyping
MH  - Interleukin-4/biosynthesis
MH  - Male
MH  - Monocytes/*immunology
MH  - Recombinant Proteins/biosynthesis
MH  - Sheep/*immunology
PMC - PMC1782798
EDAT- 2002/11/09 04:00
MHDA- 2002/12/17 04:00
PMCR- 2003/11/01
CRDT- 2002/11/09 04:00
PHST- 2002/11/09 04:00 [pubmed]
PHST- 2002/12/17 04:00 [medline]
PHST- 2002/11/09 04:00 [entrez]
PHST- 2003/11/01 00:00 [pmc-release]
AID - 1515 [pii]
AID - 10.1046/j.1365-2567.2002.01515.x [doi]
PST - ppublish
SO  - Immunology. 2002 Nov;107(3):366-72. doi: 10.1046/j.1365-2567.2002.01515.x.