PMID- 12423354
OWN - NLM
STAT- MEDLINE
DCOM- 20030114
LR  - 20190620
IS  - 0014-2956 (Print)
IS  - 0014-2956 (Linking)
VI  - 269
IP  - 22
DP  - 2002 Nov
TI  - Role of three isoforms of phospholipase A2 in capacitative calcium influx in 
      human T-cells.
PG  - 5557-63
AB  - The present study was conducted on human Jurkat T-cell lines in order to 
      elucidate the role of phospholipase A2 in capacitative calcium entry. We have 
      employed thapsigargin (TG) that induces increases in [Ca2+]i by emptying the 
      calcium pool of endoplasmic reticulum, followed by capacitative calcium entry. We 
      designed a Ca2+ free/Ca2+ reintroduction (CFCR) protocol for the experiments, 
      conducted in Ca2+-free medium. By employing CFCR protocol, we observed that 
      addition of exogenous arachidonic acid (AA) stimulated TG-induced capacitative 
      calcium influx. The liberation of endogenous AA and its autocrine action seems to 
      be implicated during TG-induced capacitative calcium influx: TG potentiates the 
      induction of constitutively expressed mRNA of four PLA2 isoforms (type 1B, IV, V, 
      VI), the inhibitors of the three PLA2 isotypes (type 1B, V, VI) inhibit 
      TG-induced release of [3H]AA into the extracellular medium, and finally, these 
      PLA2 inhibitors do curtail TG-stimulated capacitative calcium entry in these 
      cells. These results suggest that stimulation of three isoforms of PLA2 by 
      thapsigargin liberates free AA that, in turn, induces capacitative calcium influx 
      in human T-cells.
FAU - Hichami, Aziz
AU  - Hichami A
AD  - UPRES Lipides & Nutrition, Universite de Bourgogne 21000 Dijon, France; Central 
      Jalma Research Institute for Leprosy, Agra, UP, India.
FAU - Joshi, Beenu
AU  - Joshi B
FAU - Simonin, Anne Marie
AU  - Simonin AM
FAU - Khan, Naim Akhtar
AU  - Khan NA
LA  - eng
PT  - Journal Article
PL  - England
TA  - Eur J Biochem
JT  - European journal of biochemistry
JID - 0107600
RN  - 0 (Protein Isoforms)
RN  - 0 (RNA, Messenger)
RN  - 27YG812J1I (Arachidonic Acid)
RN  - 67526-95-8 (Thapsigargin)
RN  - EC 3.1.1.32 (Phospholipases A)
RN  - EC 3.1.1.4 (Phospholipases A2)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Arachidonic Acid/metabolism
MH  - Calcium/*chemistry/metabolism
MH  - Electrophysiology
MH  - Humans
MH  - Jurkat Cells
MH  - Phospholipases A/antagonists & inhibitors/*chemistry/metabolism
MH  - Phospholipases A2
MH  - Protein Isoforms
MH  - RNA, Messenger/metabolism
MH  - Signal Transduction
MH  - T-Lymphocytes/*metabolism
MH  - Thapsigargin/pharmacology
EDAT- 2002/11/09 04:00
MHDA- 2003/01/15 04:00
CRDT- 2002/11/09 04:00
PHST- 2002/11/09 04:00 [pubmed]
PHST- 2003/01/15 04:00 [medline]
PHST- 2002/11/09 04:00 [entrez]
AID - 3261 [pii]
AID - 10.1046/j.1432-1033.2002.03261.x [doi]
PST - ppublish
SO  - Eur J Biochem. 2002 Nov;269(22):5557-63. doi: 10.1046/j.1432-1033.2002.03261.x.